脂肪细胞分化相关基因在大鼠再生肝中表达变化(英文)  

EXPRESSION PROFILES OF THE GENES RELATED TO ADIPOCYTE DIFFERENTIATION IN THE RAT REGENERATING LIVER

作　　者：赵利峰[1] 邵恒熠[2] 徐存拴 

机构地区：[1]中国海洋大学生命科学与技术学部 [2]河南师范大学生命科学学院,新乡453002

出　　处：《分子细胞生物学报》2007年第3期224-232,共9页Journal of Molecular Cell Biology

基　　金：973计划前期研究专项(No.2006CB708506)~~

摘　　要：肝脏由多种细胞构成,肝再生与细胞分化密切相关,细胞分化受基因转录水平调控。为在基因转录水平了解脂肪细胞分化基因在大鼠肝再生中作用,本文用搜集网站资料和查阅相关论文等方法获得上述基因,用Rat Genome2302.0芯片检测它们在大鼠肝再生(liver regeneration,LR)中表达情况,将三次检验结果相同或相似、在肝再生中表达变化2倍以上、真手术组和假手术组相比差异显著的基因视为肝再生相关基因。初步证实上述基因中75个基因与肝再生相关。肝再生启动(PH后0.5-4h)、G0/G1过渡(PH后4-6h)、细胞增殖(PH后6-66h)、细胞分化和组织结构功能重建(PH后72-168h)等四个阶段起始表达的基因数为44、13、30和1;基因的总表达次数为88、58、302和90。表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们共表达上调313次、下调167次,分为43种表达方式。表明肝再生中脂肪细胞发生和分化相关基因活动多样和复杂。根据本文研究结果推测,上述基因不仅调节脂肪细胞分化,而且参与肝再生的生理生化活动。To investigate the action of adipocyte differentiation-related genes during rat liver regeneration at transcriptional level, these genes were obtained by means of collection of the database data and retrieval of the related theses. The Rat Genome 230 2.0 array was used to inspect the expression changes of them in rat regenerating livers. Identification of the liver regeneration-associated genes was through performing three independent chip analyses, showing a greater than double change in gene expression at least at one time point during liver regeneration, and comparing differences in gene expression between partial hepatectomy （PH） and sham operation （SO）. 75 of the above genes were found to be liver regeneration-related. In initiation phase of liver regeneration （0.5-4 h after PH）, G0/G1 （4-6 h after PH）, cell proliferation （6-66 h after PH）, cell differentiation and liver tissue structure-function reconstruction （72-168 h after PH）, the number of the initially expressed geneswas 44, 13, 30 and 1 respectively, and the total expression times of the genes were in a sequence of 88, 58, 302 and 90, illustrating that the initially expressed genes were advantaged in initial phase （0.5-4 h）, and yielded function in each phases. The genes were totally up-regulated 313 times and down-regulated 167 times. 43 expression patterns of them conferred multiformity and complication on the cellular physiological and biochemical activities liver regeneration involving. The results indicated that the above genes not only can regulate the adipocyte differentiation, but also can participate in the physiological and biochemical activities during liver regeneration.

关 键 词：部分肝切除 Rat GENOME 230 2.0芯片 脂肪细胞分化 肝再生相关基因 

分 类 号：Q343[生物学—遗传学]