电针对脑缺血再灌注大鼠缺血区皮质TNF-α mRNA表达及TNF-α含量影响  

The Effects of Electroacupuncture on the Expression of TNF-α mRNA and the Level of TNF-α in Ischemic Penumbra in Cortices of Rats with Cerebral Ischemia-Reperfusion

作　　者：丁德光[1] 李家康[1] 罗惠平[1] 周仲愉[1] 焦扬[1] 韦丹[1] 

机构地区：[1]湖北省中医院,武汉430061

出　　处：《中国中医急症》2007年第6期695-697,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：湖北省教育厅科研基金资助项目(No.2004D006)

摘　　要：目的通过观察电针对脑缺血再灌注大鼠缺血区皮质TNF-α mRNA的表达和TNF-α含量的影响以探讨电针对缺血性脑损伤的保护机制。方法采用大脑中动脉线栓法制作模型;电针大鼠的水沟、内关,运用逆转录-多聚酶链反应(RT-PCR)法检测缺血区皮质TNF-α mRNA表达和放射免疫法检测皮质TNF-α含量。结果针刺各组缺血皮质中TNF-α mRNA表达和TNF-α含量与模型组相比均降低,有统计学意义。结论电针通过抑制TNF-α mRNA的表达减少TNF-α合成,以对抗缺血再灌注脑组织神经损伤。Objective： To observe the effect of electroacupuncture（EA） on the expression of TNF -α mRNA and the level of TNF -α in ischemic penubra in cortices of rats with ischemia - reperfusion, so that probe the mechanism of EA against cerebral ischemia injury. Methods： EA on rats＇ Shuigou（GV26） and Neiguan（P6）. Measured the level of expression of TNF -α mRNA by RT- PCR, the level of TNF -α in cortices by radioimmunoassy（RIA）. Results： The expression of TNF -α mRNA and the level of TNF -α in cortex of EA groups were lower, comparing with those of the model groups. Conclusion： EA can lighten the neurons injury after cerebral ischemia- repeffusion by decreasing the expression of TNF -α mRNA in the ischemia region and the creation of TNF -α in cortex.

关 键 词：脑缺血 再灌注损伤 电针 肿瘤坏死因子-α 大鼠 

分 类 号：R245.319[医药卫生—针灸推拿学]