碱性成纤维细胞因子在博莱霉素致肺纤维化肺血管损伤中的作用  

The role of bFGF in vascular injury of BLM-induced pulmonary fibrosis

作　　者：刘华[1] 陈利萍[2] 朱新菊 贺永锋[4] 赵杰[4] 刘新社[2] 

机构地区：[1]北京市公安局,北京100085 [2]西安交通大学医学院法医系,陕西西安710061 [3]镇江市公安局刑侦支队,江苏镇江212000 [4]陕西省公安厅刑侦局,陕西西安710016

出　　处：《西安交通大学学报（医学版）》2007年第3期273-275,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省自然科学基金资助项目(No.2003C238)

摘　　要：目的探讨碱性成纤维细胞因子(bFGF)在博莱霉素(BLM)致大鼠肺纤维化肺组织中的表达及其在肺血管损伤中的作用。方法于大鼠气管内注射BLM以复制其肺纤维化模型,应用bFGF多克隆抗体按SABC法行免疫组织化学染色,定量图像分析。结果BLM组动物支气管上皮细胞胞浆内、支气管和肺小动脉肌层以及肺泡巨噬细胞胞浆内可见较多棕黄色细颗粒状bFGF阳性表达产物,显著高于对照组(P<0.05)。结论在BLM致大鼠肺纤维化过程中bFGF表达上调,提示bFGF可能参与肺间质纤维化的发病过程。Objective To explore the expression of basic fibroblast growth factor（bFGF） in pulmonary tissues of rats with bleomycin-induced pulmonary fibrosis and the role of bFGF in vascular injury. Methods Pulmonary fibrosis was induced in rats by intratracheal injection of bleomycin（BLM）. Lung expression of bFGF proteins was assessed by SABC immunohistochemistry using bFGF monoclonal antibody and quantitative image analysis. Results The expression of bFGF in the lung tissues of BLM group was mainly distributed in bronchiole epithelial cells, muscular layers of bronchi and arterioles, and alveolar macrophage. It was higher than that in control group, with significant difference （P 〈 0. 05）. Conclusion The up-regulation expression of bFGF in the BLM-induced pulmonary fibrosis indicates that it directly participates in the process of pulmonary fibrosis.

关 键 词：碱性成纤维细胞因子 博莱霉素 肺纤维化 血管损害 免疫组化 

分 类 号：R563.1[医药卫生—呼吸系统]