依曲替酸对角质形成细胞肝素结合表皮生长因子样生长因子mRNA的影响  

Effect of acitretin in inducing the expression of heparin-binding epidermal-growth-factor-like growth factor mRNA in normal human keratinocytes

作　　者：罗素菊[1] 彭振辉[1] 郑焱[1] 张路坤[1] 王蔚[2] 王国荣[3] 

机构地区：[1]西安交通大学医学院第二附属医院皮肤科,陕西西安710004 [2]西安交通大学医学院第二附属医院泌尿外科,陕西西安710004 [3]陕西省人民医院普通外科,陕西西安710068

出　　处：《西安交通大学学报（医学版）》2007年第3期319-321,333,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30371295;30400387);陕西省自然科学基金资助项目(No.2004C211)

摘　　要：目的研究依曲替酸对培养的正常人角质形成细胞(KC)增殖和肝素结合表皮生长因子样生长因子(HB-EGF)mRNA表达的影响。方法0.1和1μmol/L伊曲替酸处理正常人KC 12 h后,用噻唑蓝比色法(MTT法)和实时荧光定量逆转录聚合酶链反应(RT-PCR)法分别检测KC增殖和HB-EGF mRNA表达的改变。结果依曲替酸处理12 h后,可剂量依赖抑制KC的增殖和上调HB-EGF mRNA的表达。0.1和1μmol/L伊曲替酸分别使KC增殖抑制10.2%和14.4%,使HB-EGF mRNA增加到正常对照组的3.2和7.1倍。与对照组相比,差异均有统计学意义(P<0.05)。结论依曲替酸可剂量依赖上调HB-EGF mRNA的表达,其可能参与依曲替酸对KC增殖的抑制作用。Objective To investigate the alteration of cell proliferation and heparin-binding epidermal-growth-factor-like growth factor（HB-EGF）mRNA expression in cultured normal human keratinocytes after acitretin treatment. Methods After a 12-hour incubation with 0. l and 1μmol/L acitretin in normal human keratinocytes, the proliferation potency of the cells was detected by MTT colorimetric assay and the expression of HB-EGF mRNA was examined by real-time quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results Both keratinocytes growth inhibition and HB-EGF mRNA expression was dose-dependently induced by acitretin. Treatment with 0. l and 1μmol/L acitretin inhibited keratinocyte proliferation by 10.2% and 14.4%, and elevated HB-EGF mRNA by 3.2-and 7.1-fold, respectively. Conclusion Upregulated HB-EGF mRNA expression induced by acitretin in normal human keratinocytes may be involved in regulating keratinocyte growth inhibition after acitretin treatment

关 键 词：表皮生长因子 角质形成细胞 维甲酸 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]