治带片中苦参碱及氧化苦参碱的HPLC法测定  

Determination of Alkaloids in Zhidaipian Tablets by high performance liquid chromatogram

作　　者：胡森科[1] 于燕[1] 苗江丽[1] 

机构地区：[1]西安交通大学医学院公共卫生系,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2007年第3期343-345,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的建立治带片中苦参碱及氧化苦参碱的高效液相色谱(HPLC)测定方法。方法色谱柱:Lichrospher-NH2(4.6 mm×250 mm,5μm),C18保护柱;流动相:乙腈-无水乙醇-0.5 mol/L磷酸水溶液(80∶10∶10);检测波长212 nm;流速1.0 mL/min;柱温:室温;进样量20μL。结果苦参碱和氧化苦参碱线性范围均为1.0-10.0μg/mL,回归方程苦参碱为:C=1.201×10-4A+0.161,r=0.9992;氧化苦参碱为:C=1.366×10-4A+0.221,r=0.9996,平均回收率分别为99.9%和99.4%,RSD分别为1.48%和4.33%。结论本法简便快捷,结果准确,可用于该制剂的质量控制。Objective To establish a method of high performance liquid chromatogram （HPLC） for measuring matrine and oxymatrine in Zhidaipian Tablets. Methods A Lichrospher-NH2 column （4.6 mm × 250mm, 5μm） was used with a mobile phase consisting of acetonitrile-ethanol-0.5 mol/L phosphoric acid （80 ： 10 ： 10）, and in a flow rate of 1.0 mL/min. The detective wavelength was 212 nm. Column temperature was room temperature and the injection quantity was 20μL. Results The samples were well separated. The linear range was the same for the target （1.0-10.0μg/mL）. The linear equations for matrine wasC = 1. 201 × 10^-4A ＋0. 161 , the correlation coefficient was 0. 9992, and for oxymatrine was C = 1. 366 × 10^-4A ＋ 0. 221 , while the correlation coefficient was 0. 9996, respectively. The mean recovery rate were 99.9% and 99.4%, respectively. RSD was 1.48% and 4.33 %, respectively （ n = 6）. Conclusion The method is simple, reliable, accurate and can be used to the quality control of the Zhidaipian Tablets.

关 键 词：治带片 苦参碱 氧化苦参碱 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]