柱后光化学衍生荧光检测高效液相色谱法测定2种新型肟醚类农药  

Determination of Two Novel Oxime Insecticides by High Performance Liquid Chromatography with Post-column Photochemical Derivatization and Fluorescence Detection

作　　者：刘晓伟[1] 梁婧[2] 温裕云[2] 张金城[2] 弓振斌[1] 

机构地区：[1]厦门大学近海海洋环境科学国家重点实验室,厦门361005 [2]厦门大学海洋与环境学院,厦门361005

出　　处：《分析化学》2007年第6期901-904,共4页Chinese Journal of Analytical Chemistry

基　　金：福建省自然科学基金重点项目(No.0220001);厦门大学科技创新工程基金(No.XDKJCX20051005)资助项目

摘　　要：建立了柱后光化学衍生荧光检测高效液相色谱法测定2种新型肟醚类农药（硫肟醚、HNPC-A2005）的方法.在优化的流动相配比（V甲醇∶ V水=80∶ 20）及流速（1.0 mL/min）条件下,用配备ZORBAX SB-C18色谱柱、自制的光化学反应器及荧光检测器的高效液相色谱仪对待测组分进行分离和测定.在最佳条件下,方法的检出限分别为0.056 mg/L（硫肟醚）、0.036 mg/L（HNPC-A2005）;线性范围为0.090～5.0 mg/L;相对标准偏差（0.1 mg/L, n=8）分别为3.5%（硫肟醚）、0.8%（HNPC-A2005）.A high performance liquid chromatographic method with post-column photochemical derivatization and fluorescence detection was developed for the determination of two novel insecticides, sulfur oxime and HNPC-A2005. Two insecticides were separated by ZORBAX SB-C18 column and eluant of methanol/water （ Vmethanol： Vwater = 80：20） at flow rate of 1.0 mL/min and then measured by a fluorescence detector after photochemical defivatization with a laboratory-built photochemical reactor. Under optimized conditions, detection limits（ S/N = 3） of sulfur oxime and HNPC-A2005 were 0. 056 mg/L and 0. 036 mg/L, respectively. Linear calibration curves were obtained in the range of 0. 090 - 5.0 mg/L for the two insecticides. Relative standard deviations were 3.5% for sulfur oxime and 0. 8% for HNPC-A2005 （0. 1 mg/L, n =8）.

关 键 词：柱后光化学衍生 荧光检测 高效液相色谱 肟醚类农药 

分 类 号：TQ450.7[化学工程—农药化工] O657.72[理学—分析化学]