60个玉米自交系的SSR标记分析  

The SSR Markers Analysis of 60 Maize Inbred Lines

作　　者：李凌雨[1] 孟全业[2] 王学雄[1] 闫彩清[1] 王圆荣[2] 马玉光[2] 

机构地区：[1]山西省农业科学院作物遗传研究所,山西太原030031 [2]农业部农作物种子质量监督检验测试中心(太原),山西太原030006

出　　处：《华北农学报》2007年第3期25-29,共5页Acta Agriculturae Boreali-Sinica

基　　金：山西省回国留学人员基金项目(2001068);山西省自然科学基金项目(20031080)

摘　　要：利用SSR标记研究了60个山西省主要玉米（Zea maysL.）自交系的遗传变异。用23对扩增带型稳定的引物,从供试自交系中检测出91个等位基因变异,每对引物检测出等位基因2-7个,平均为3.96个,平均多态性信息含量为0.600。UPGMA聚类分析将60个自交系划分为唐四平头,旅大红骨,PN,BSSS,Lancaster,Suwan等6个类群,化群结果与其系谱关系和育种家经验基本一致。Simple sequence repeats （SSRs） markers were used to detect genetic variation of 60 elite maize inbred lines. Twenty-three SSR primers giving stable amplified detected 96 alleles among the inbred lines tested. The average number of allele per SSR locus was 3.96 with an range from 2 to 7 .The value of polymorphism information content（PIC） for each SSR locus varied from 0.15 to 0.80 with an average of 0.600.The UPGMA analysis classified 60 inbred lines into 6 clusters, which corresponed to the heterotic groups determined by their pedigree information and breeder＇s experiences（i, e. Tangsipingtou, Luda Red Cob, PN, BSSS, Lancaster, Suwan）

关 键 词：SSR标记 玉米 自交系 

分 类 号：S513.03[农业科学—作物学]