大鼠死后骨骼肌FTIR光谱变化与死亡时间的关系  

Examination of Rat's Skeletal Muscle Degradation with Fourier Transform Infrared Spectroscopy to Estimate Postmortem Interval

作　　者：黄平[1] 刘勇[2] 柯咏[1] 鲁庆阳[1] 邢博[1] 樊拴良[1] 王振原[1] 

机构地区：[1]西安交通大学医学院法医学系,陕西西安710061 [2]西安市公安局未央分局,陕西西安710016

出　　处：《法医学杂志》2007年第3期164-166,169,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30471935);西安交通大学博士基金资助项目(N160071206)

摘　　要：目的应用傅立叶变换红外光谱(FTIR)分析大鼠死后骨骼肌随死亡时间推移的化学降解过程,为死亡时间推断提供新的研究方法。方法大鼠断颈处死后置于(20±2)℃环境,不同的死亡时间点提取大鼠大腿骨骼肌组织,并运用FTIR光谱仪测定不同化学基团随死亡时间的变化。结果随着死亡时间的推移,大鼠骨骼肌组织不同吸收峰的峰强随着死亡时间增加呈现出四种不同的变化方式:增加、下降、稳定、波动,且不同峰强比显示了相似的时间变化趋势。结论FTIR光谱分析技术有望成为法医实践中推断死亡时间的新方法。Objective To study postmortem degradation in rat＇s skeletal muscle with fourier transtorm intrared spectroscopy （FTIR） and explore a new method to estimate postmortem interval （PMI）. Methods Rats were sacrificed by cervical dislocation and the bodies were kept in a chamber set at （20±2） ℃. FTIR spectra of rat＇s skeletal muscle were measured at the different postmortem time points from 0 to 144 h. Results The FTIR absorbance bands at different FTIR wave length （cm^-1） showed the four different type changes： increase, decrease, stable, and fluctuation. The various bands intensity ratio also demonstrated the similar changes to the relative bands intensity. Conclusion FTIR spectroscopy may be a new effective method to estimate the PMI in forensic practice.

关 键 词：傅立叶变换红外光谱(FTIR) 死亡时间 骨骼肌 化学基团 

分 类 号：R339.14[医药卫生—人体生理学]