固定化胰蛋白酶活力的测定方法探讨  

Study on Determination of the Activity of Immobilized Trypsin

作　　者：库汉生[1] 姚晓玲[1] 张婷[1] 刘丹松[1] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068

出　　处：《氨基酸和生物资源》2007年第2期33-35,共3页Amino Acids & Biotic Resources

基　　金：湖北省教育厅科学技术研究项目(项目编号2004D003)

摘　　要：对自制的固定化胰蛋白酶(Immobilization Trypsin,ITP)分别经过冷冻干燥、真空干燥后定量测定其酶活力,与由湿态直接定量法(DQA)测定的活力进行比较。试验表明,直接定量方法测定的酶活力比前两者高,而且操作简单。The directly quantitative analysis （DQA） methods to measure the activity of the immobilized trypsin were studied, and were compared to measurement on the activity of the immobilized trypsin dried by freeze or vacuum. The results showed that the activity on DOA was higher than that on the others.

关 键 词：固定化 胰蛋白酶 活力回收率 

分 类 号：Q55[生物学—生物化学]