薄层色谱法分离紫杉醇和三尖杉宁碱  

Separation of Taxol and Cephalomannine by Thin Layer Chromatography

作　　者：董慧茹[1] 雒丽娜[1] 郑云 赵京城 

机构地区：[1]北京化工大学理学院,北京100029 [2]北京普瑞孚天然药物现代纯化和分离研究所,北京100088

出　　处：《理化检验（化学分册）》2007年第6期472-475,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：建立了用薄层色谱分离紫杉醇和三尖杉宁碱的方法。方法中以正己烷-二氯甲烷-甲醇-三乙胺(体积比4.5∶3.5∶0.5∶1.0)四元体系为展开剂,在硅胶薄层板上成功地分离了紫杉醇和三尖杉宁碱,所得斑点清晰、无拖尾。同时以该体系为展开剂,使用薄层色谱法指导分离提取紫杉醇和三尖杉宁碱过程中反相色谱流分的合并,方法快速、简单、成本低,取得了满意的结果。In the method of separation of taxol and cephalomannine by thin layer chromatography, a solution containing n-hexane, dichloromethane, methanol and triethylamine （mixed in the ratio of 4. 5 ： 3. 5 ： 0. 5 ： 1.0） was used as developing solvent （mobile phase） and the silica gel thin layer plate was used as the stationary phase. By using the modified mobile phase, taxol and cephalomannine were separated satisfactorily, the spots were separated clearly, sharply and without trailing.

关 键 词：薄层色谱 紫杉醇 三尖杉宁碱 三乙胺 

分 类 号：O658.1[理学—分析化学]