垫扎球囊法复制大鼠在体心肌缺血/再灌注模型  

Experimental Model of Myocardial Ischemia/reperfusion in Rat Established by Ligating Coronary Artery with Capsule

作　　者：赵秀梅[1] 孙胜[1] 刘秀华[1] 

机构地区：[1]中国人民解放军总医院病理生理学研究室,北京100853

出　　处：《中国微循环》2007年第3期206-208,共3页Journal of Chinese Microcirculation

基　　金：国家自然科学基金(30370569);国家自然科学基金重大国际合作(30620130111)资助项目

摘　　要：目的 优化大鼠在体心脏缺血/再灌注模型。方法 雄性SD大鼠，采用气管插管、人工呼吸、开胸、冠状动脉左前降支上垫扎充水球囊阻断血流的方法造成心肌缺血，通过塌陷气囊进行心肌再灌注，复制大鼠心肌缺血/再灌注模型。结果垫扎充水球囊阻断血流后1-2min内出现急性心肌缺血的心电图改变，从颈动脉灌注1%依文思蓝，冠状动脉左前降支供血区不蓝染，气囊塌陷恢复血流后整个心脏被依文思蓝蓝染，模型复制成功率为97%。结论 垫扎球囊法复制大鼠在体心肌缺血/再灌注模型，操作简便易行，成功率高。Objective To improve the experimental model of myocardial isehemia/reperfusion （I/R） in rats. Methods Under anesthesia and artificial ventilation, the thorax was opened and a capsule was placed on the left anterior descending coronary artery（LAD）. The myocardial ischemia was performed by ligating LAD with a water-filled capsule with a 6-0 silk suture attached to an atraumatic needle. The reperfusion was performed by empting the capsule to re-open the LAD. Results The electrocardiogram showed acute ischemic alteration at 1 - 2 min after LAD occlusion by water-filled capsule, the risk area was free from stain by Evans Blue, indicating the complete occlusion of LAD. The unstained myoeardium was stained by Evans Blue as soon as the capsule was flattened, indicating that LDA was completely re-opened. Condusion The improved experimental I/R model in rat myoeardium is an easy-operating and reliable method.

关 键 词：心脏 缺血/再灌注 动物模型 大鼠 

分 类 号：R331.31[医药卫生—人体生理学]