鼠尾草酸联合1,25-二羟基维生素D_3诱导HL-60细胞分化作用的研究  

The differentiation of HL-60 cells induced by 1,25-dihydroxyvitamin D_3 combined with carnosic acid

作　　者：安慧萍[1] 任立红[1] 陈娟娟[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院儿内科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2007年第3期237-240,共4页Journal of Harbin Medical University

摘　　要：目的研究鼠尾草酸与1,25-二羟基维生素D3[1,25(OH)2D3]联合应用对HL-60细胞分化的影响。方法通过四唑氮蓝(MTT)法观察细胞生长,光学显微镜观察细胞形态,应用流式细胞仪测定细胞周期及成熟单核细胞分化标记CD14的表达,观察联合应用鼠尾草酸与低浓度1,25(OH)2D3对HL-60细胞的影响。结果低浓度1,25(OH)2D3(1nmol/L)抑制HL-60细胞增殖、诱导细胞分化的作用与对照组比较无统计学意义(P>0.05)。同浓度1,25(OH)2D3与10μmol/L鼠尾草酸联合处理HL-60细胞72h后,细胞形态具有成熟单核细胞特征、细胞增殖明显受抑制、细胞阻滞于G0/G1期、CD14的表达率为57.624%;其作用效应与对照组比较有显著性差异(P<0.01),与单独应用高浓度1,25(OH)2D3(100nmol/L)组比较作用相似,无统计学意义(P>0.05)。结论鼠尾草酸可增强1,25(OH)2D3对HL-60细胞的诱导分化、抑制增殖作用。Objective To study the effects of 1,25-dihydmxyvitamin D3 combined with carnosic acid on differentiation of human acute promylocytic leukemia （APL） cell line HL-60. Methods The proliferation rates of HL-60 cells were exaimined by MTT assay. The changes of morphologic features of HL-60 cells were observed by microscope; cell cycle parameters and monocytic differentiation marker CD14 were detected by flow cytometry.Results Treated with lower concentrations of 1,25（OH）2D3 （1nmol/L）, the proliferation of HL-60 cells could not be inhibited and the expression of CD14 showed no notable difference compared with that of control group.Treated using 1,25 （OH）2D3 （1nmol/L） combined with CA （10μmol/L） for 72 hours, HL-60 cells showed mature monocytic features, the proliferation of cells was inhibited and cell cycle arrested in G0/G1 phase,the expression of CD14 was 57. 624% ;The effects had more significant difference than that of control group but no statistic difference compared with high concentrations （100nmol/L） of 1,25（OH）2D3 group. Conclusion Carnosic acid can enhance the differentiation and inhibit the proliferation of HL-60 cells induced by 1, 25（OH）2D3.

关 键 词：细胞分化 1 25(OH)2D3 鼠尾草酸 HL-60细胞 

分 类 号：Q813.1[生物学—生物工程]