巴戟天多糖及其水提取物对体外培养成骨细胞活性的影响  

Effects of morinda root polysaccharide and its extract on the activity of osteoblast cultured in vitro

作　　者：李楠[1] 王和鸣[1] 郭素华[2] 林旭[3] 郑良朴[4] 王力[1] 

机构地区：[1]福建中医学院骨伤系,福建省福州市350108 [2]福建中医学院药学系,福建省福州市350108 [3]福建医科大学分子医学中心,福建省福州市350004 [4]福建中医学院中西医结合研究院,福建省福州市350108

出　　处：《中国组织工程研究与临床康复》2007年第23期4570-4572,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(30271630);福建省青年科技人才创新项目(2003J039)~~

摘　　要：目的:体外培养成骨细胞,观察巴戟天多糖和巴戟天水提物对成骨细胞活性的影响。方法:实验于2004-09/2005-08在福建省骨伤研究所骨伤分子生物学实验室完成。①提取24h内新生SD大鼠头盖骨成骨细胞,分别用50,100,200g/L巴戟天多糖及50,100,200g/L巴戟天水提物药物血清进行体外培养。对照组用含50,100,200g/L无药血清的DMEM培养液培养。培养72h后进行相关指标检测。②通过MTT方法检测巴戟天多糖、巴戟天水提取物对成骨细胞增殖的影响。③检测碱性磷酸酶、细胞内骨钙素、转化生长因子β1基因表达等指标,观察巴戟天多糖、巴戟天水提取物对成骨细胞活性的影响。结果:①药物血清对成骨细胞增殖的影响:100g/L巴戟天水提物组和100g/L巴戟天多糖组吸光度大于对照组,差异有非常显著性(P<0.01),且100g/L巴戟天多糖组吸光度高于100g/L巴戟天水提物组(0.4302±0.0193,0.3829±0.0228,P<0.01)。②药物血清对成骨细胞活性的影响:巴戟天水提物组与巴戟天多糖组碱性磷酸酶比活性、细胞内骨钙素含量、转化生长因子β1mRNA相对表达量均高于对照组[碱性磷酸酶比活性:(16.89±2.09),(20.68±2.44),(11.23±2.40)μkat/g;骨钙素含量:(3.74±0.82),(3.97±0.69),(2.63±0.34)ng/106;转化生长因子β1 mRNA相对表达量:2.97±0.37,4.23±0.30,0.07±0.27,P均<0.01]。巴戟天多糖碱性磷酸酶比活性、转化生长因子β1 mRNA相对表达量高于巴戟天多糖组(P<0.01)。结论:巴戟天水提物与巴戟天多糖均能显著促进体外培养成骨细胞增殖、促进成骨细胞分泌碱性磷酸酶与骨钙素、促进成骨细胞转化生长因子β1 mRNA的表达,并且巴戟天多糖优于巴戟天水提物。AIM： To observe the effect of morinda root polysaccharide and its aqueous extract on the activity of osteoblast cultured in vitro. METHODS： The experiment was conducted in the molecular biological laboratory of Fujian Orthopaedics and Traumatology Institute from September 2004 to August 2005.①Osteoblasts were extracted from the parietal bone of SD rats bom in 24 hours, and cultured in drug serum of 50, 100, 200 g/L morinda root polysaccharide and 50, 100, 200 g/L aqueous extract, respectively. The control group was cultured in DMEM culture solution with 50, 100 and 200 g/L drug free serum. Seventy-two hours later, the related indexed were detected, ②The effects of morinda root polysaccharide and its aqueous extract on the proliferation of osteoblasts were detected by MTT.③The alkaline phosphatase lAP）, osteocalcin （OC） and the expression of transforming growth factor β1 （TGF-β1） were also measured. RESULTS：① Effect of drug serum on osteoblast proliferation： The absorbances of the 100 g/L morinda root polysaccharide group and 100 g/L morinda root group were higher than that of the control group （P 〈 0,01）, and that of the morinda root polysaccharide group was higher than the morinda root group （0.430 2±0.019 3, 0.382 9±0.022 8, P 〈 0.01 ）, Effect of drug serum on the osteoblast activity： The AP, OC and expression of TGF-β1 of the morinda root polysaccharide group and morinda root group were higher than those of the control group lAP： （16.89±2.09）, （20.68±2.44）, （11.23±2.40） μkat/g; OC： （3.74±0.82）, （3.97±0.69）, （2.63±0.34） ng/10^th, TGF-β1 mRNA： （2.97±0.37, 4.23±0.30, 0.07＋0.27）, P 〈 0.01]. Moreover, those of the modnda root polysaccharide group were higher than the morinda root group （P〈 0.01）, CONCLUSION： Both morinda root polysaccharide and morinda root could promote the proliferation of osteoblasts, secretion of alkaline phosphatase and osteocalcin, and expression of transforming growth factor β1, Moreover, t

关 键 词：巴戟天多糖 成骨细胞 大鼠 碱性磷酸酶 骨钙素 转化生长因子Β1 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]