胎盘早剥产妇、新生儿血及脐血中组织因子和组织因子途径抑制物的变化  

Changes of tissue factor and tissue factor pathway inhibitor in blood of placental abruption puerperants and newborns and cord blood

作　　者：唐雯[1] 杨红玲[2] 冯琼[2] 陶少华[1] 石小东[1] 花少栋[1] 孙学刚[3] 封志纯 

机构地区：[1]南方医科大学珠江医院儿科,广东省广州市510515 [2]广州市妇婴医院,广东省广州市510180 [3]南方医科大学中医药学院中医症候实验室,广东省广州市510282 [4]北京八一儿童医院,北京市100700

出　　处：《中国组织工程研究与临床康复》2007年第24期4791-4793,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:检测胎盘早剥产妇血、新生儿血及脐血组织因子及组织因子途径抑制物蛋白的变化,以及这些变化对其凝血功能的影响。方法:收集2006-10/2007-04在中山大学附属第一医院产科的胎盘早剥产妇血(足月或早产不限)、分娩胎儿的脐带血及新生儿血标本各11例,4例为足月产,其余均为胎龄29～36周早产标本,男婴5份,女婴6份。正常对照15份来自同期广州市妇婴医院产妇及分娩胎儿。标本收集时间母亲血为分娩前24h内、脐血为分娩即刻、新生儿血为出生后2h内抽取,排除标准为产妇本次分娩前无血液系统疾病史,未使用过对凝血功能产生影响的药物,采用酶联免疫吸附方法测定血浆组织因子及组织因子途径抑制物抗原水平,并与正常分娩产妇对照组进行比较。病理及正常标本均经产妇及家属知情同意后获得。结果:①胎盘早剥产妇血、新生儿血及脐血血浆中组织因子测定值明显高于正常对照组(P<0.05)。②胎盘早剥产妇血、新生儿血浆及脐血中组织因子途径抑制物测定值较正常分娩产妇降低,差异具有显著性意义(P<0.01)。③胎盘早剥产妇血、新生儿和脐血组织因子途径抑制物/组织因子比值均较正常分娩产妇明显降低(P<0.01)。结论:胎盘早剥时产妇血、脐血及新生儿血促凝血活性增强,抗凝血活性降低,与其高凝状态相关。AIM： To investigate the effects of levels of tissue factor （TF） and tissue factor pathway inhibitor （TFPI） proteins in blood of placental abruptfon （PA） puerperants and newborns and cord blood on their blood clotting function. METHODS： From October 2006 to April 2007, 11 PA puerperant blood samples, 11 fetal cord blood samples and 11 newbom blood samples were enrolled at First Affiliated Hospital of Sun Yet-sen University, and 4 cases were term birth, others with fetal age of 29-36 weeks, including 5 male infant blood samples and 6 female infant blood samples. The 15 control samples at the same time ware collected from puerperants and newborns of Guangzhou Women and Children＇s Hospital. The samples were gotten 24 hours before labor from mothers, at the time of delivery from cord blood and 2 hours after birth. These puerperants were neither having the history of hematological system diseases nor using drugs that might affect the coagulation function. The plasma levels of TF and TFPI antigen were measured by enzyme linked immunosorbent assay （ELISA）. Pathological data and normal samples were collected with the agreement of puerperants and their family members. RESULTS： ①The plasma level of TF antigen in PA puerperants, newborns and cord blood were significantly higher than the normal controls （P 〈 0.05）. ②The levels of TFPI in PA puerperants, newborns and cord blood were significantly decreased （P 〈 0.01 ）. ③The same results of the ratio TFPI/TF were obviously lower than the normal controls in the PA puerperants （ P 〈 0.01 ）. CONCLUSION： The precoagulant activitities are enhanced in PA puerperants, newborns and cord bloods, and the anti-procoagulant activities are decreased. These are associated with hypercoagulable state.

关 键 词：组织因子 组织因子途径抑制物 胎盘早剥 新生儿 产妇 

分 类 号：R714.56[医药卫生—妇产科学]