酶联免疫吸附法检测心力衰竭模型大鼠尿液水通道蛋白2的变化及意义(英文)  

Change and significance of urinary excretion of aquaporin-2 detected by enzyme linked immunosorbent assay in rat models of heart failure

作　　者：卢武生[1] 许顶立[2] 孟素荣[2] 陈政良[3] 

机构地区：[1]解放军第一七五医院心血管科,福建省漳州市363000 [2]解放军第一军医大学南方医院心血管内科,广东省广州市510515 [3]解放军第一军医大学基础部免疫学教研室,广东省广州市510515

出　　处：《中国组织工程研究与临床康复》2007年第25期5042-5043,共2页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:通过酶联免疫吸法检测不同严重程度充血性心力衰竭模型大鼠尿液水通道蛋白2浓度的变化,并与假手术组进行比较。方法:实验于2000-01/2002-01在解放军第一军医大学南方医院动物实验室完成。选用雄性成年SD大鼠42只。取大鼠26只,采用左冠状动脉结扎制备慢性心力衰竭大鼠模型。以左心室梗死面积≥20%为充血性心力衰竭模型造模成功(心力衰竭组,n=13),而左心室梗死面积<20%为心肌梗死心功能代偿组(代偿组,n=13)。其余16只大鼠为对照组:未行冠状动脉结扎。采用BeckmanC×3仪器测定血清钠浓度,采用冰点抑制法测定尿渗量,监测24h尿量。采用双抗体夹心酶联免疫吸附法测定大鼠尿水通道蛋白2浓度。结果:进入结果分析数量保持为42只。①心力衰竭组和代偿组大鼠24h尿量和血钠浓度明显低于对照组(P<0.05～0.01),尿渗量明显高于对照组(P<0.05～0.01)。②对照组大鼠术后4和6周尿液水通道蛋白2水平明显低于其他2组(P<0.05～0.01),代偿组明显低于心力衰竭组(P<0.05,0.01),代偿组和心力衰竭组术后6周明显高于4周(P<0.05)。结论:①水通道蛋白2是心力衰竭时水潴留和低钠血症的关键靶蛋白。②酶联免疫吸附法检测尿液水通道蛋白2浓度,可以有效反映心力衰竭时水潴留和低钠血症状况。AIM： To detect the change of urinary concentration of aquaporin-2 （AQP-2） by enzyme linked immunosorbent assay in rat models of different degrees of heart failure and make a comparison with sham-operation group. METHODS： This experiment was carried out between January 2000 and January 2002 in the animal laboratory of Nanfang Hospital of Southern Medical University. Forty-two male adult Sprague-Dawley rats were involved. Twenty-six rat models of chronic heart failure were prepared by ligation of left coronary artery. When left ventricle infarct area was≥20%, the rat models of congestive heart failure were successful （heart failure group, n =13）; When left ventricle infarct area was〈20%, the rat models of congestive heart failure were unsuccessful （compensation group, n =13）. The other 16 rats were not ligated at coronary rtery （control group）. Serum sodium concentration was determined with BeckmanC×3 equipment and urine osmole by cryoscopic method. Urine volume of 24 hours was monitored. Urinary concentration ofAQP-2 level of rats was determined by double antibodies sandwich enzyme linked immunosorbent assay （DABs-ELISA）. RESULTS： Forty-two rats were involved in the result analysis. The 24-hour urine volume and serum sodium concentration in the heart failure group and compensation group were significantly lower than those in the control group （P〈0.05-0.01）, while urine osmole in two groups was significantly higher than that in the control group （P〈0.05-0.01）.②At postoperative 4 and 6 weeks, urinary concentration of AQP-2 level of rats in the control group was significantly lower than that in the other two groups （P〈0.05-0.01）, and urinary concentration of AQP-2 level of rats in the compensation group was significantly lower than that in the heart failure group （P〈0.05, 0.01）.In the compensation group and heart failure group, urinary concentration of AQP-2 level of rats was significantly higher at postoperative 6 weeks than at postoperative 4 weeks （P〈0

关 键 词：水通道蛋白2 酶联免疫吸附测定 充血性心力衰竭 低钠血症 

分 类 号：R541.6[医药卫生—心血管疾病]