大鼠肠屏障功能变化与不同强度游泳运动的关系(英文)  

Relationship between intestinal barrier function and swimming exercise of different intensities

作　　者：史艳莉[1] 费曦艳[2] 

机构地区：[1]武汉工业学院健康科学与护理系,湖北省武汉市430023 [2]武汉大学医学院,湖北省武汉市430072

出　　处：《中国组织工程研究与临床康复》2007年第25期5058-5060,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:研究在运动应激状态下肠道屏障损伤发生机制及规律,将为运动应激肠道屏障保护剂的研制提供理论依据。目的:观察不同强度游泳运动后大鼠肠屏障的变化。设计:随机对照动物实验。单位:湖北大学运动人体科学实验室和武汉大学医学院基础实验室。材料:选取3周龄健康雄性SD大鼠36只,随机分为对照组10只、适度运动组12只和过度性运动组14只,3组大鼠饲养条件一致。方法:①对照组平时不运动。②适度运动组:进行无负重游泳,前3d适应性游30min,并在1周内逐渐延长至60min,然后每天游泳1次,每周6次,训练6周。③过度运动组:前3d适应性游30min,并在1周内延长时间至120min,训练1周后,进行过度游泳训练。然后每天游泳1次,每周6次,持续4周。最后2周,每天早、晚1次,每周6次。主要观察指标:①大鼠肠屏障参数:血浆内毒素,肠黏膜通透性,细菌移位率。②大鼠肠黏膜组织结构。结果:①大鼠肠屏障参数结果:大鼠过度运动后血浆内毒素增加2倍,肠黏膜通透性提高2.5倍,细菌移位率增加230%。②适度运动对大鼠肠黏膜组织结构无明显影响,过度运动使大鼠肠上皮细胞内高尔基复合体和粗面内质网扩张,上皮细胞高度水肿,炎细胞浸润。结论:适度运动可以改善机体肠功能;过度运动造成机体肠屏障损伤,导致消化系统出现病理性征候群。BACKGROUND; Studying the pathogenesis and rule of intestinal barrier damage under exercise stress will provide theoretical evidence for preparing intestinal barrier protectant under this state. OBJECTIVE ： To observe the changes in intestinal barrier of rats following different intensities of swimming exercise. DESIGN： Randomized controlled animal experiment. SETTING： Laboratory of Exercise Sciences ＆ Sports Medicine, Huibei University; Basic Laboratory, Medical College, Wuhan University. MATERIALS： Thirty-six healthy male SD rats, aged 3 weeks, were involved and randomized into 3 groups： control group （n =10）, proper exercise group （n =12） and excessive exercise group （n =14）. The rats of three groups were raised in the same condition. METHODS：①Control group： Rats did not exercise normally. ②Proper exercise group： Rats swam without loading. In the first 3 days, they adaptatively swam for 30 minutes and gradually for 60 minutes within 1 week, then they swam once a day, 6 times a week, 6 weeks in total. ③Excessive exercise group： In the first 3 days, they adaptatively swam for 30 minutes and gradually for 120 minutes within 1 week. After trained for 1 week, they were given excessive swimming training. Then, they were forced to swan once a day, 6 times a week, within 4 weeks successively. Within later 2 weeks, the rats were forced to swan once in the morning and evening separately, 6 times a week. MAIN OUTCOME MEASURES：①Intestinal barrier parameter： intestinal mucosal permeability, plasma endotoxin, becterium shift rate.②Intestinal mucosal membrane structure. RESULTS： Thirty-six rats were involved in final analysis.①After excessive exercise, plasma endotoxin of rats was doubly increased, intestinal mucosal permeability was enhanced by 2.5 times, and bacterium shift rate was increased by 230%.②Proper exercise had no obvious influences on the structure of intestinal mucosal membrane tissue of rats, and excessive exercise expanded Golgi complex in the intesti

关 键 词：过度运动 肠屏障 内毒素 细菌移位 

分 类 号：R339.4[医药卫生—人体生理学]