用顶体反应释放的顶体酶活性评价顶体反应功能的研究  

Study on evaluating acrosome reaction function by acroin activity released acrosome reaction

作　　者：王洪亮[1] 刘睿智[2] 张红国[3] 赵丁[2] 许宗革[2] 

机构地区：[1]吉林大学第一医院泌尿外科,130021 [2]吉林大学基础医学院细胞生物学教研室 [3]东北师范大学遗传与细胞研究所

出　　处：《中国妇幼保健》2007年第18期2576-2579,共4页Maternal and Child Health Care of China

摘　　要：目的:研究用顶体反应释放的顶体酶活性评价人类精子顶体反应功能。方法:10例健康生育男子精液,采用Percoll密度梯度分离法优选精子,用FITC-PSA荧光染色法分析顶体反应(acrosome reaction,AR),顶体酶活性采用分光光度比色法测定。结果:A23187和孕酮诱导的精子AR释放的顶体酶活性和顶体反应率均明显增加(P<0.05)。随A23187浓度增加,AR释放的顶体酶活性和顶体反应率均明显增加(P<0.01)。A23187诱导AR释放的顶体酶活性与相应顶体反应率呈明显正相关关系(r=0.950,P<0.001)。随孕酮浓度增加,AR释放的顶体酶活性和顶体反应率均明显增加(P<0.05)。孕酮诱导的AR释放的顶体酶活性与相应顶体反应率亦呈明显正相关关系(r=0.921,P<0.001)。结论:孕酮可诱导人精子顶体反应,用顶体反应释放的顶体酶活性可评价人精子顶体反应功能。Objective： To investigate acrosome reaction function by acroin activity released acrosome reaction. Methods： Human semen was obtained from 10 fertile donors with normal semen parameters. Specimens were allowed to liquefy at 37℃, and were prepared by Pereoll - gradient treatment. Spermatozoa were recovered from the upper layer. Acrosome reaction was analyzed using FITC - PSA staining. Acrosin activity was analyzed using the colorimetric by spectrocolorimeter. Results： When calcium ionosphere A23187 and progesterone induced acrosome reaction, the percentage of acrosome reacted sperm and released acrosin activity were both markedly increased（P 〈 0. 05 ）. With increase of final concentration of A23187, the percentage of acrosome reacted sperm and released acrosin activity were both markedly increased （P 〈 0. 01 ） . There was a markedly positive relative between the percentage of acrosome reacted sperm and released acrosin activity （r =0. 950, P 〈0. 001 ） . With increase of final concentration of progesterone, the percentage of acrosome reacted sperm and released acrosin activity were both markedly increased （ P 〈 0. 05 ） . There was a markedly positive relative between the percentage of acrosome reacted sperm and released acrosin activity （ r = 0. 921, P 〈 0. 001 ） . Conclusion： When progesterone induced acrosome reaction, released acrosin activity can evaluate this sperm function.

关 键 词：人类精子 顶体反应 顶体酶活性 孕酮 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]