益气活血法对脑出血大鼠血肿区血管新生的形态学影响  

The Effect of Yi-Qi Huo-Xue Therapy on Angiogenesis of Brains in Rats with Intracerebral Hemorrhage

作　　者：齐勇[1] 唐涛[1] 罗杰坤[1] 邢之华[1] 张宗棨[1] 虢灿杰[1] 王玲[1] 刘小娟[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,长沙410008

出　　处：《中国康复》2007年第3期150-152,共3页Chinese Journal of Rehabilitation

基　　金：国家自然科学青年基金(30400581);中国博士后科学基金(2005038224);湖南省青年骨干教师培养对象经费(湘教通[2005]64号);湖南省重点学科建设经费(湘教通[2001]179号)

摘　　要：目的:观察益气活血法对脑出血大鼠脑内损伤区血管新生和血肿吸收的影响及作用机制。方法:180只SD大鼠分为正常组5只,假手术组35只,另140只利用胶原酶诱导建立大鼠脑出血模型后分为模型组、益气组(益气方灌胃)、活血组(活血方灌胃)及益气活血组各35只,不同时间点灌注取脑。采用脑微血管银染法观察各组血管新生情况、测量血肿面积。结果:与正常组及假手术组比较,第1天时,造模的4组大鼠血肿内可见大量结构不清的液化坏死脑组织、炎性细胞浸润,内皮细胞肿胀,血肿周围少量呈毛细血管扩张,中线移位。与造模后的其它3组比较,造模后第7天益气活血组血肿开始明显吸收,35d时部分切片完全吸收(P<0.01)。第14-28天时血肿周围及内部新生血管增多。第28天后各组新生血管开始消退。结论:益气活血法可能通过提高血肿区巨噬细胞吞噬功能,增强血管新生和血肿吸收,以促进脑组织修复。Objective： To investigate the mechanisms of Yi-Qi Huo-Xue therapy （YQHX） by observing its effects on angiogenesis and hematoma absorption of brains in rats with intracerebral hemorrhage. Methods： ICH model was induced by injecting collagenase type Ⅶ. Sprague-Dawley rats were randomly divided into 6 groups, including normal control group, sham group, ICH model group, Yiqi-treated group, HuoXue-treated group and YQHX-treated group. Animals were randomly chosen to be perfused with paraformaldehyde at the postoperative day 1, 4, 7, 14, 21, 28 and 35. Angiogenesis was observed by argentation. Image pro plus 5.0 software was used to measure the areas of hematomas. Results： Argentation revealed during 14-28 days, YQHX-treated group had more new vessels surrounding and inside hematomas; New vessels began to regression in all groups at 28th day. The areas of hematoma were largest in model group and HuoXue-treated group at first day. The hematoma in YQHX-treated group was observed more quickly than in other groups during 7--35 days （P〈0.01） and disappeared in some slices at 35th day. Conclusion： The YQHX therapy could accelerate the absorption of hematoma and promote cerebral repair of ICH rats, which might be related with the increased phagotrophic function of macrophages and enhanced angiogenesis.

关 键 词：益气活血 脑出血 血肿 微血管 大鼠 

分 类 号：R277.7[医药卫生—中医学]