^(60)Co γ辐照对冬小麦幼苗保护酶同工酶表达的影响  

Effects of ^(60)Co γ ray irradiation on protective enzymes isozymes expression in winter wheat

作　　者：戚微娜[1] 吕金印[2] 赵军[2] 高俊凤[2] 

机构地区：[1]西北农林科技大学理学院,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2007年第7期123-126,130,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：西北农林科技大学校重点科研项目

摘　　要：为了探讨60Coγ射线的诱变机理,用0,100,300,500 Gy剂量60Coγ射线对陕合6号和长武131 2个小麦品种的种子进行辐照处理,并采用垂直板聚丙烯酰胺凝胶电泳对二叶一心期小麦叶片和根中的过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)同工酶进行了分析。结果表明,2个品种的小麦种子经100 Gy60Coγ辐照处理后,其酶谱带数与对照相比变化不大;经300和500 Gy60Coγ辐照处理后,小麦叶片中CAT和SOD同工酶谱带消失,POD同工酶谱带仍然存在,且增加了4条谱带;小麦根中3种保护酶同工酶谱带数均有不同程度增加,POD同工酶增加了1条Rf值为0.411的谱带,CAT同工酶增加了2条Rf值分别为0.488和0.524的谱带。以上结果说明,小麦根较叶片对辐射更加敏感。In order to define the mutated mechanism, two different genotypes seed of Shaanhe 6 and Changwu 131 were adopted, and the seeds were radiated by ^60Coγ ray under different doses of 0,100,300, and 500 Gy,respectively. When the wheat grows to the period of three leaves cultured with water,and then peroxidase （POD）, catalase （CAT） and superoxide dismutade （SOD） isozymes of wheat were analyzed. The results showed that the isozymes changed little when radiated by 100 Gy doses compared with control. But CAT and SOD isozymes disappeared in the leaves, POD still existed, and increased 4 bands when radiated by 300 and 500 Gy doses. But bands of three kinds of isozymes in the roots had to different degree increased.isozymes of POD increased a band of Rf 0. 411 ,and CAT increased two bands of Rf 0. 488 and Rf 0. 524,which means the roots are more sensitive to radiation than the leaves.

关 键 词：^60CO 小麦 过氧化物酶同工酶 过氧化氢酶同工酶 超氧化物歧化酶同工酶 

分 类 号：S512.110.352[农业科学—作物学]