钙离子参与一氧化氮促进盐胁迫下的玉米种子萌发  

Ca^(2+) Involved in Seed Germination of Maize (Zea mays L.) Induced by Nitric Oxide under Salt Stress

作　　者：唐静[1] 韩宇[1] 陈康[1] 王玉图[1] 刘新[1] 

机构地区：[1]莱阳农学院生命科学学院,山东青岛266109

出　　处：《植物生理学通讯》2007年第3期421-424,共4页Plant Physiology Communications

基　　金：山东省教育厅基金(J04C13);国家自然科学基金(30370141)

摘　　要：0.001～1.0 mmol·L^(-1)一氧化氮(NO)供体硝普钠(SNP)均能提高玉米种子的发芽率,缓解盐胁迫下种子萌发的抑制作用,其中0.1 mmol·L^(-1)SNP的效果最佳。用胞外游离Ca^(2+)螯合剂EGTA、质膜Ca^(2+)通道阻断剂LaCl_3和液泡Ca^(2+)释放抑制剂钌红与0.1 mmol·L^(-1) SNP共处理,均能减弱或抵消SNP促进种子萌发的作用。据此推测,钙离子参与SNP促进盐胁迫下玉米种子萌发的信号转导过程。0.001-1.0 mmol·L^-1 sodium nitroprusside （SNP）, an exogenous nitric oxide （NO） donor, could increase the seed germination percentage of maize （Zea mays） and relieve the inhibition of salt stress to seed germination. The effect of 0.1 mmol.L^-1 SNP was the best. After treatment of extracellular dissociation Ca^2＋ chelant EGTA, in combination with blocker of plasma membrane Ca^2＋ channels LaCl3 and inhibitor of vacuole Ca^2＋release ruthenium red, the effect of SNP was decreased. Our results suggested that Ca^2＋ was involved in the signal transduction of maize seed germination induced by SNP under salt stress.

关 键 词：钙离子 一氧化氮 盐胁迫 玉米 种子萌发 

分 类 号：S513[农业科学—作物学] Q945.78[生物学—植物学]