盐胁迫下朝鲜碱茅的甜菜碱醛脱氢酶活性变化及其基因保守区的克隆  

Change in Betaine Aldehyde Dehydrogenase Activity of Puccinellia chinampoensis Ohwi and Cloning of Its Conserved cDNA Region under Salt Stress

作　　者：杨铮[1] 钟鸣[1] 郭志富[1] 李丽[1] 陈罡[2] 

机构地区：[1]沈阳农业大学辽宁省农业生物技术重点实验室,沈阳110161 [2]辽宁省林业科学研究院生物中心,沈阳110032

出　　处：《植物生理学通讯》2007年第3期430-434,共5页Plant Physiology Communications

基　　金：辽宁省重点实验室专项资金;辽宁省博士启动基金(辽宁省科委2001102061)

摘　　要：文章探讨了朝鲜碱茅甜菜碱醛脱氢酶活性在盐胁迫下的变化,用简并引物扩增了甜菜碱醛脱氢酶基因保守区序列的结果表明,该保守区段长438 bp,推测编码145个氨基酸,包括醛脱氢酶高度保守序列V[T/S]LELGGKSP和其后29位与酶功能有关的Cys。此序列Genbank登录号为EF095710。Change in betaine aldehyde dehydrogenase （BADH） activity of Puccinellia chinampoensis under sail stress was explored and the conserved sequence of BADH was amplified using degenerate primers. The results demonstrated that the conserved sequence was 438 bp in length, which was deduced coding 145 amino acids. The deduced products contained the conserved motif of aldehyde dehydrogenase which was V[T/S]LELGGKSP and the Cys associated with aldehyde dehydrogenase function, The gene accession nucleotide sequence number in GenBank was EF095710.

关 键 词：朝鲜碱茅 盐胁迫 甜菜碱醛脱氢酶(BADH) 简并引物 保守区 同源性 

分 类 号：S543.9[农业科学—作物学] Q943.2[生物学—植物学]