温度对枳椇次生胚发生和植株再生的影响  

Effects of Temperature on Secondary Somatic Embryogenesis and Plant Regeneration in Hovenia dulcis Thunb.

作　　者：李成浩[1] 刘宝光[1] 王伟达[1] 刘立琨[1] YU Chang-Yeon 

机构地区：[1]东北林业大学黑龙江省林木遗传育种重点实验室教育部林木遗传育种与生物技术重点实验室(在建),哈尔滨150040 [2]江原大学农业生命科学学院

出　　处：《植物生理学通讯》2007年第3期453-456,共4页Plant Physiology Communications

基　　金：国家自然科学基金(30671701);黑龙江省博士后基金

摘　　要：在含有2,4-D的培养基上所诱导和增殖的枳棋体细胞胚只能形成无再生能力的畸形胚。通过次生胚发生途径可以在不加植物生长调节物质的培养基诱导出发育正常的体细胞胚,子叶期胚的次生胚诱导能力高于早期体胚。相对高的温度(30℃)有利于次生胚的诱导,相对低的温度(20℃)更有利于次生胚的发育、植株再生和移栽成活。Somatic embryos induced on medium containing 2,4-dichlorophenoxyacetic acid, could only develop abnormal embryos. Well developed somatic embryos could be obtained through secondary somatic embryogenesis in medium without plant growth regulator. The induced ability of cotyledonary somatic embryos induced more secondary embryos was higher than early stage somatic embryos. The temperature strongly affected on secondary embryogenesis, relatively high temperature （30℃） was effective for induction of secondary somatic embryos, and low temperature （20℃） was more suitable for further embryo development, plantlet regeneration and transplantation survival.

关 键 词：次生胚发生 重复体胚发生 培养温度 植物生长调节物质 

分 类 号：Q943.1[生物学—植物学]