通心络体外促进人外周血内皮祖细胞的增殖  

Tongxinluo enhancing proliferation of peripheral blood-derived human endothelial progenitor cells

作　　者：梁小卫[1] 孙承波[1] 王华[1] 梁春[1] 吴宗贵[1] 

机构地区：[1]第二军医大学长征医院心内科,上海200003

出　　处：《第二军医大学学报》2007年第6期598-602,共5页Academic Journal of Second Military Medical University

基　　金：国家重点基础研究发展计划("973"计划)(2005CB523309).~~

摘　　要：目的:观察中药通心络(Tongxinluo)对体外培养的人外周血内皮祖细胞(endothelial progenitor cells,EPCs)增殖的影响。方法:采用密度梯度离心法分离培养人外周血单个核细胞,经FITC标记荆豆凝集素Ⅰ(FITC-UEA-Ⅰ)和Dil标记的乙酰化低密度脂蛋白(Dil-acLDL)双染色鉴定为正在分化的EPCs,并进一步通过流式细胞仪检测其表面标志CD34、CD133。分别以不同浓度(0、50、100、200、500、750和1 000μg/ml)通心络超微粉溶液作用36 h,以及500μg/ml通心络超微粉溶液作用不同时间(0、6、12、24和36 h)后.MTT法观察EPCs增殖的情况。结果:与对照组比较,不同浓度通心络组均改善了EPCs的增殖功能(P<0.05),在500μg/ml时最为显著(P<0.01);500μg/ml通心络能明显促进EPCs增殖能力(P<0.05,P<0.01),随时间延长促进作用逐渐增强,36 h达到高峰(增殖率为54.18%,P<0.01)。结论:通心络体外能显著改善外周血内皮祖细胞增殖的能力。Objective：To study the effects of Tongxinluo,a traditional Chinese medicine, on the proliferation of peripheral blood-derived endothelial progenitor cells （EPCs） in vitro. Methods： The Tongxinluo solution was prepared through ultrasonication according to the pervious literature. The mononuclear cells （MNCs） were isolated from the peripheral blood with Ficoll by density gradient centrifugation. MNCs were suspended in Medium 199 containing 20% fetal blood serum （FBS） and vascular endothelial growth factor（VEGF）. After cultured for 7 d, the attached cells were characterized by Di-LDL uptaking and FITC-lectin binding by laser confocal microscope, and further identified through detection of CD34 and CD133 expression by flow cytometry. Then the cultured EPCs were incubated with Tongxinluo at a series of concentrations （0, 50, 100, 200, 500, 750, 1 000μg/ml） for different durations （0, 6, 12, 24 and 36 h）. The cell morphology was observed and cell proliferation was determined by MTT assay. Results： Incubation with different concentrations of Tongxinluo increased the proliferative ability of EPCs. Tongxinluo at 500μg/ml had the most prominent effect on proliferation and the effect increased as time went by and reached peak at 36 h （growth rate 54.18%, P%0.01）. Conclusion： Tongxinluo can greatly improve the proliferation of EPCs in viiro.

关 键 词：通心络 内皮祖细胞 细胞增殖 

分 类 号：R972.6[医药卫生—药品]