LD_(50)剂量芥子气中毒对大鼠生殖系统的影响  

Effect of mustard gas at LD50 dose on reproductive system of rats

作　　者：尚丽新[1] 裴丽鹏[1] 张黎明[2] 李巨[1] 申健[1] 

机构地区：[1]解放军第202医院妇产科,沈阳110003 [2]第二军医大学海军医学系防化医学教研室,上海200433

出　　处：《第二军医大学学报》2007年第6期637-639,共3页Academic Journal of Second Military Medical University

基　　金：全军"十五"指令性课题(01L054).~~

摘　　要：目的:评价芥子气中毒对大鼠生殖系统的影响。方法:成年SD大鼠80只.随机分为中毒组和对照组.每组40只,雌雄各半。中毒组背部皮下注射芥子气(5 mg/kg.LD_(50).溶于丙二醇中),对照组注射等量丙二醇,分别于1、3、5、7和28 d每次取雌、雄各3只从眼球取静脉血.采用放免试剂盒检测血清中雌二酵、睾酮、卵泡刺激素(FSH)、黄体生成素(LH)的浓度。取血后处死动物.取其生殖器官(卵巢或睾丸)光镜下观察组织学改变。于中毒后第4天及第5天死亡雌鼠2只,雄鼠3只。结果:雌性大鼠的组织学及血清学改变均在一定时期内恢复正常。雄性大鼠在染毒后1个月.病理结果仍显示生精细胞大部分坏死脱落,曲细精管呈空网状;同时血清FSH水平从对照组的(1.210 0±0.354 6)mmol/L升高至染毒28d后的(1.475 0±0.045 1)mmol/L,差异具有显著性(P<0.01)。结论:芥子气对雌性大鼠生殖系统的损害较轻.且恢复较快,而对雄性大鼠的生殖系统却存在着持久而不可逆的损害。Objective：To evaluate the effect of mustard gas on reproductive system in rats. Methods： Forty male SD rats and 40 female rats were equally divided into mustard gas-treated group and control group. Animals in mustard gas-treated group were injected with 5 mg · kg body weight （LD50） of mustard gas, and those in control group received propylene glycol injection. Serum hormonal determinations （estriol,testosterone, luteinizing hormone, follicle-stimulating hormone） were completed by collecting ocular vein blood at 1 day, 3 days, 5 days, 7 days and 28 days after injection. The rats were then killed were anatomized. Microscope was used to observe pathological changes in germ cells （ovary or testes）. Two female rats and 3 male rats died during 4th and 5th day after injection. Results： The changes of serum hormone and pathology nearly restored to the normal levels in the female rats after injection of mustard gas for a short period. Most of the spermatogenic cells were still necrotic and seminiferous tubules still had hollow cross 1 month after injection in male rats. The FSH levels in the sera of male rats were significangtly increased from （1.2100±0.3546） mmol/L in control group to （ 1.4750±0.0451） mmol/L 28 days after injection（P〈0.01）. Conclusion： Mustard gas causes slight injury of ovary in female rats and the damage can recover in a short period,but it can cause long-time and unreversable injury to testes in male rats.

关 键 词：芥子气 卵巢 睾丸 性腺激素类 

分 类 号：R827.1[医药卫生—临床医学]