普鲁泊福对黄疸大鼠离体心功能及氧自由基代谢的影响  

Effects of propofol on isolated heart function and metabolism of oxygen free radical in rats with obstructive jaundice

作　　者：任红梅[1] 任安经[2] 徐学武[1] 郭志福[3] 杨立群[1] 俞卫锋[1] 

机构地区：[1]第二军医大学东方肝胆外科医院麻醉科,上海200438 [2]第二军医大学基础部生理学教研室,上海200433 [3]第二军医大学长海医院心血管内科,上海200433

出　　处：《第二军医大学学报》2007年第6期640-643,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30600584).~~

摘　　要：目的:研究临床浓度的普鲁泊福对黄疸大鼠离体心脏心功能及氧自由基代谢的影响。方法:36只SD大鼠随机分为假手术(SO)组和胆总管结扎(BDL)组(n=18)。离体心脏灌流30 min平衡后各组随机取6只心脏测定基础丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力。两组所余动物再各自随机分为2个亚组(n=6):Krebs-Henseleit液(K-H液)灌流组和普鲁泊福灌流组。K-H液灌流组为K-H液继续灌流40 min,普鲁泊福灌流组用含有25μmol/L普鲁泊福的K-H液继续灌流40 min。灌流稳定后记录各组心率(HR)及左心室功能指标,灌流结束后测定心脏组织MDA含量、SOD活力。结果:BDL组心功能指标基础水平均显著低于SO组(P<0.05)。两组心功能指标普鲁泊福灌流后较灌流前显著下降(P<0.05).而各指标变化率未见显著差异。BDL组MDA含量基础值显著高于SO组(P<0.05),而SOD活力基础值显著低于SO组(P<0.05);普鲁泊福灌流后BDL组MDA含量较灌流前显著降低(P<0.05).而SOD活力显著升高(P<0.05)。结论:临床浓度普鲁泊福对黄疸大鼠离体心功能的影响与正常大鼠相比并不显著,这可能与普鲁泊福保护黄疸大鼠的SOD活力、降低MDA,改善心肌代谢的作用有关。Objective：To study the effects of propofol of clinical concentration on isolated heart function and metabolism of oxygen free radicals in rats with obstructive jaundice. Methods： Thirty-six rats were randomly divided into 2 groups （n=18） ： sham operated group （SO） and bile duct ligation group （BDL）. The isolated working hearts were perfused for 30 min and 6 hearts were randomly selected from each group to measure the contents of basic MDA and SOD activity in the heart tissue. The rest animals of each group were randomly separated into 2 subgroups （n = 6）：K-H solution perfusion group and propofol perfusion group. The isolated working hearts in K-H solution perfusion group were perfused with K-H solution for 40 min. The hearts in propofol perfusion group （SOP, BDLP） were perfused for 40 min with K-H solution containing 25 μmol/L propofol. After the hearts became stable, the following indices were recorded： HR, left ventricular developed pressure （LVDP）, ±dp/ dtmax, etc. The contents of MDA and SOD activity were measured after perfusion. Results： After perfusion with K-H solution for 30 min, the basic indices of heart function of BDL group were significantly lower than those of SO group （P〈0.05）. The indices significantly decreased after perfusion with propofol （P〈0.05）, but the rates of change of all indices were not different between SO and BDL groups. The contents of basic MDA in BDL groups were significantly higher than those of SO groups （P〈0.05）; the basic SOD activities in BDL were significantly lower than those of SO groups （P〈0. 05）. After propofol perfusion, MDA content of BDLP group significantly decreased （P〈0.05） and SOD activity significantly increased （P〈0.05）. Conclusion： Propofol at clinical concentration shows no significantly different effects on isolated working heart function of rats with and without obstructive jaundice, which might be related to the fact that propofol can protect SOD activity, lower MDA content and imp

关 键 词：梗阻性黄疸 普鲁泊福 心功能 丙二醛 超氧化物歧化酶 

分 类 号：R971.2[医药卫生—药品]