药物与蛋白质相互作用研究方法的进展  

Drug and protein interactions:progress of research methods

作　　者：姜虹[1,2] 安普丽[1] 蒋晔[1] 

机构地区：[1]河北医科大学药学院分析化学教研室 [2]白求恩军医学院训练部仪器中心,石家庄050081

出　　处：《第二军医大学学报》2007年第6期662-666,共5页Academic Journal of Second Military Medical University

摘　　要：药物和蛋白质的相互作用是生命科学研究的重要领域,本文综述了近5年相关研究的技术和方法,包括光谱分析法、平衡透析法、超滤法、微透析法、毛细管电泳法、核磁共振、X射线晶体衍射技术、电化学法、亲和层析法、迎头分析法、液相色谱法等。目前研究表明往往需多种方法联合使用才能阐明特定药物与其靶蛋白的相互作用。Interaction between drug and protein is a major research field of life science. This article reviews the methods used in related research in the past 5 years, including spectroscopy, equilibrium dialysis, ultrafiltration, microdialysis method, capillary electrophoresis, NMR, X-ray diffraction, electrochemical, affinity chromatography, HPFA, HPLC, etc. It has been showed that combined usages of several technologies are always needed to elucidate an interaction between drug and protein.

关 键 词：药物 蛋白质 相互作用 

分 类 号：R96[医药卫生—药理学]