脑震荡对大鼠脑内乙酰胆碱N受体表达的影响  

Effect of Brain Concussion on the Expression of N Acetylcholine Receptor in Rats

作　　者：李坪[1] 于建云[2] 郭泽云[1] 许冰莹[2] 赵小林[2] 兰光明[2] 

机构地区：[1]昆明医学院组织胚胎学教研室,云南昆明650031 [2]昆明医学院法医学院,云南昆明650031

出　　处：《昆明医学院学报》2007年第3期1-4,共4页Journal of Kunming Medical College

基　　金：国家自然科学基金资助(30160027;30560041);云南省自然科学基金资助(2005C0026Q)

摘　　要：目的探讨脑震荡（BC）后认知行为障碍的大鼠中枢烟碱型N受体（N-R）的变化规律.方法应用金属单摆式机械打击装置复制单纯性脑震荡大鼠模型,采用免疫组织化学结合图像分析的方法,研究BC后大脑前额叶皮质（PFC）、海马、丘脑、尾壳核（CPU）和Broca斜角带垂直支（VDB）5个脑区N受体蛋白的表达情况.结果N受体蛋白在海马CA1-4、丘脑、PFC、VDB和CPU有阳性表达,伤后虽呈轻度下降变化,但与正常组相比无显著性差异（P〉0.05）.结论推测BC后N-R蛋白的轻微下调与BC认知功能改变之间可能不存在联系.Objective To explore the changes of N acetylcholine receptor in rats with cognitive disorder after brain concussion （BC）. Methods The brain concussion was induced by a metallic pendulum -striker concussive device in rats. The changes of nicotine acetylcholine receptor （N -R） in PFC, CA1-4, Thalamus, CPU and VDB were studied by immunohistochemistry and image analysis. Results The results showed that the expression of N receptor in CA1 -4, Thalamus, PFC, CPU and VDB declined slightly after injury （ P 〉 0. 05）. Conclusion It suggested that there might be no certain relationship between the slight down regulation of N acetylcholine receptor in relative cerebral region with the changes of cognitive function after brain concussion.

关 键 词：脑震荡 烟碱型N受体 大鼠 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]