引种栽培品药用大黄中蒽醌类成分分析  

Analysis of Anthraquinones in cultivated Rheum officinale Baill

作　　者：康毅华[1] 陈智岩[1] 王振月[1] 王谦博[2] 唐先明[1] 周哓慧[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江省药品检验所,黑龙江哈尔滨150010

出　　处：《中医药学报》2007年第3期30-31,共2页Acta Chinese Medicine and Pharmacology

摘　　要：目的:分析引种栽培品药用大黄中蒽醌类成分的情况。方法:采用高效液相色谱法及薄层色谱法测定,色谱柱为ThermoC18柱(250mm×4.6mm),流动相为甲醇:0.1%磷酸水=70:30;柱温:室温;流速:1ml/min;检测波长:254nm。展开剂:石油醚(60～90℃)-甲酸乙酯-甲酸(15:8:1)的上层溶液;检测波长:365nm。结果:引种栽培品药用大黄中蒽醌类成分含量高于原产地甘肃药用大黄对照药材。Objective: To analysis the Anthraquinone content in cultivated Rheum officinale Baill. Method: The HPLC and TLC methods were employed. The samples were separated by isocratic elution on Thermo-C18 Hypersil column (250mm×4.6mm,5μm),and then, with the methanol-0.1% phosphoric acid solution as mobile phase, detected wave at UV254nm and the flow rate 1.0ml/min. The developing agent was upper stratum liquor of petroleum benzin (60～90℃)- ethyl formate-formic acid(15:8:1).The samples were detected at UV365nm.Results: The Anthraquinone contents of cultivated Rheum officinale Baill. exceeded the standard sample of Rheum officinale Baill. from Gansu Province.

关 键 词：栽培品药用大黄 蒽醌类成分 高效液相色谱法 薄层色谱法 

分 类 号：R927.2[医药卫生—药学]