体外培养人眼睫状肌细胞的流体阻力定性观察  

Stability observation of liquid resistance of cultured human ciliary muscle cells ex vivo

作　　者：王小兵[1] 张海燕[2] 李卫红[2] 刘爱珍[1] 曾衍钧[3] 

机构地区：[1]首都医科大学附属北京同仁医院眼科中心,北京100730 [2]首都医科大学基础医学院,北京100069 [3]北京工业大学,北京100022

出　　处：《医用生物力学》2007年第2期177-180,共4页Journal of Medical Biomechanics

基　　金：国家自然科学基金(NO.10472005);北京市自然科学基金(NO.3052009)

摘　　要：目的定性观察体外培养的人眼睫状肌细胞流体阻力的稳定性,探讨作为体外实验模型的可行性。方法培养的第4代人眼睫状肌细胞传代在Transwell半透膜上,按培养8、14、20d分为三组共15份,在细胞层上下造成7mm液面差异,观察上层液体渗透至与下层液面持平所需时间。结果除第8天组不够稳定外,另两组有60%Transwell的液体流速的误差在15%以内,80%以上的误差在30%以内。结论该模型对流体的阻力是基本稳定的。Objective To observe the stability of liquid resistance of cultured human ciliary muscle cells. Methods To observe the time for 7mm high liquid to penetrate through 3 groups of 8.14.20 days cultured 4 passage human ciliary muscle cells on Transwell （5 in each group）. Results In groups of 14.20 days cells, the differences of penetrating velocity are within 15% among 60% Transwell. within 30% among 80% Transwell. Conclusion The resistance of cultured human ciliary muscle cells to liquid is almost stable.

关 键 词：睫状肌细胞 阻力 流体 

分 类 号：R318.01[医药卫生—生物医学工程]