腺病毒介导的HSV-TK对骨关节炎滑膜细胞的作用  

Effect of the HSV-TK gene with adenovirus mediation on osteoarthritic synoviocytes

作　　者：丁远景[1] 张伟[1] 李伟[1] 张磊[1] 孙水[1] 王健[1] 王先泉[1] 

机构地区：[1]山东大学山东省立医院关节外科,山东济南250021

出　　处：《山东大学学报（医学版）》2007年第6期590-594,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30672115)

摘　　要：目的:观察腺病毒介导的单纯疱疹病毒-胸苷激酶基因(HSV-TK)对骨关节炎滑膜细胞的作用。方法:含HSV-TK基因的重组腺病毒转染骨关节炎滑膜细胞,观察转染效率,经丙氧鸟苷(GCV)处理后测定滑膜细胞的抑制率,动态观察细胞形态学变化,荧光染色观察细胞核形态学变化,定量分析细胞的凋亡和坏死率。结果:含TK基因的重组腺病毒有效感染骨关节炎滑膜细胞,治疗组对滑膜细胞有显著的抑制作用(P<0.05),转染细胞死亡有坏死和凋亡两种形式。结论:HSV-TK/GCV系统对骨关节炎滑膜细胞有显著的抑制作用。Objective： To investigate the effect of the HSV-TK gene on adenovirus-mediation on osteoarthritic synoviocytes. Methods： Cultured osteoarthritic synoviocytes were transduced with the adenovirus containing the HSV-TK gene. The expression of the adenovirus was determined. Cells without treatment served as the controls and the infected cells were treated with GCV. Then the inhibition ratio was determined. The preliminary morphological changes were observed by light microscopy and the nucleus subjected to fluorescent staining was observed by fluorescent microscopy. The proportion of necrosis and apoptosis was quantitatively analyzed. Results： The adenovirus had the ability to infect the cultured osteoarthritic synoviocytes. The proliferation of the treatment group was significantly inhibited （ P 〈 0.05）. Necrosis and apoptosis were both found in transduced cells. Conclusion： The HSV-TK/GCV system significantly inhibits the proliferation of osteoarthritic synovial cells.

关 键 词：单纯疱疹病毒属 胸苷激酶 骨关节炎 基因疗法 基因 结构 病毒 

分 类 号：R684.3[医药卫生—骨科学]