单核细胞趋化蛋白-1诱导人脐静脉内皮细胞的凋亡  

Apoptosis of human umbilical vein endothelial cells induced by monocyte chemotactic protein-1

作　　者：李琴山[1] 刘洋[1] 冯赞杰[1] 钱民章[1] 

机构地区：[1]遵义医学院生物化学教研室,贵州遵义563000

出　　处：《第四军医大学学报》2007年第12期1075-1077,共3页Journal of the Fourth Military Medical University

基　　金：贵州省科学技术基金(2003-3015)

摘　　要：目的:研究单核细胞趋化蛋白-1(MCP-1)对人脐静脉内皮细胞(hUVEC)凋亡的影响.方法:培养并鉴定hUVEC;用不同浓度MCP-1(0.1,1.0,10,100μg/L)对其作用24,48h;Annexin V/PI双染细胞,流式细胞仪观察内皮细胞凋亡;琼脂糖凝胶电泳检测细胞染色体“DNA ladder”的形成.结果:MCP-1能诱导hUVEC的凋亡,其效应随浓度和时间的增加而增强(P<0.01);Annexin V/PI显示凋亡细胞明显增加;细胞DNA呈明显的“DNA ladder”.结论:MCP-1能诱导hUVEC凋亡.AIM： To study the effects of monocyte chemotactic protein-1 （MCP-1） on the apoptosis of human umbilical vein endothelial cells （hUVEC）. METHODS： hUVEC were cultured in vitro and identified, hUVEC that growed vigorously were stimulated with different concentrations of MCP-1 （0.1, 1.0, 10, 100 μg /L）for 24, 48 hours. The early apoptotic events were detected with Annexin V/PI reagent kit by flow cytometry. Then the DNA fragments were observed by agarose gel electrophoresis. RESULTS： MCP-1 induced the apoptosis of hUVEC in a dosedependent manner and a time-dependent manner （ P 〈 0.01 ）. Annexin- V/PI double staining assay showed that stimulation to hUVEC with different concentrations of MCP-1 enhanced the apoptosis rate （P 〈 0.01 ） as compared with that in control group. The characteristic pattern of DNA ladder was observed by agarose gel electrophoresis after 24-hour treatment of MCP-1. CONCLUSION： MCP-I can induce the apoptosis of hUVEC.

关 键 词：单核细胞化学吸引蛋白质-1 脐静脉 内皮细胞 动脉硬化:细胞凋亡 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]