椒目油对大鼠气道重建的防治作用  

Effect of bunge pricklyash seed oil on airway remodeling in rats

作　　者：陈洪谦[1] 戚好文[1] 王立[1] 赵睿[1] 方丽萍[1] 

机构地区：[1]第四军医大学西京医院呼吸内科,陕西西安710033

出　　处：《第四军医大学学报》2007年第12期1091-1093,共3页Journal of the Fourth Military Medical University

摘　　要：目的:研究椒目油对哮喘大鼠模型Ⅲ型胶原、增殖细胞核抗原(PCNA)的表达水平及气道重建的影响,为椒目的临床应用提供理论依据.方法:将雄性SD大鼠30只随机分为3组:哮喘组、治疗组、对照组,每组动物10只,用卵白蛋白(OVA)腹腔注射致敏和雾化吸入激发建立哮喘大鼠模型,采用计算机图像分析免疫组化气道璧中Ⅲ型胶原的表达,并测量各组支气管壁的厚度.结果:免疫组化结果显示,Ⅲ型胶原阳性表达的平均吸光度:哮喘组大鼠(19.24±0.24)明显高于对照组(2.75±0.22,P<0.01);治疗组(10.16±0.37)明显低于哮喘组(P<0.01).PCNA的表达哮喘组明显高于对照组(P<0.05);治疗组低于哮喘组(P<0.05).哮喘组支气管壁厚度(10.24±2.05)明显高于对照组(5.81±0.73,P<0.01);治疗组(8.45±1.67)明显低于哮喘组(P<0.01).结论:椒目油不但能有效治疗哮喘,而且还能有效防止气道重建,其作用机制可能是抑制了Ⅲ型胶原和PCNA的生成.AIM： To study the effect of bunge pricklyash seed oil on the expressions of collagen type III, proliferating cell nuclear antigen （PCNA） and airway remodeling, so as to provide theoretical evidence for its clinical application. METHODS： Male SD rats （ n = 30 ） were randomly divided into 3 groups （ n = 10） ： asthma group, treatment group and control group. We made the asthma models by intraabdominal injection of ovalbumin （OVA） for allergization and OVA aerosol inhalation for challenge, then analyzed the expression of collagen type III and the thickness of airway wall by immunochemistry and image quantitative analysis methods. RESULTS： Immunochemistry of collagen type III showed that the average of asthma group（ 19.24±0. 24） was much higher than that in control group （ 2.75 ±0. 22, P 〈 0.01 ） and that in treatment group （ 10.16 ± 0.37, P 〈 0.05 ）. PCNA in asthma group was much higher than that in control group （ P 〈 0.05 ）, while that of treatment group was lower than that in asthma group（P 〈 0.05 ）. Thickness of bronchial wall in asthma group（10.24 ±2.05） was much higher than that in control group （5.81 ±0.73, P〈0.01）, and that in treatment group （8.45 ± 1.67, P 〈0.01 ）. CONCLUSION： Bunge pricklyash seed oil could effectively manage asthma and prevent airway remodeling, maybe through inhibiting the production of PCNA and collagen type III.

关 键 词：椒目油 哮喘 气道重建 增殖细胞核抗原 胶原Ⅲ型 

分 类 号：R562.25[医药卫生—呼吸系统]