Long-term application of diethylstilbestrol upregulates expressions of μ-and m-calpains in pituitary intermediate lobe of female Wistar rats  

作　　者：Weijiang Zhao Fang Yuan Guilin Li Zhongfang Shi Yun Cui Yazhuo Zhang Zhongcheng Wang 

机构地区：[1]Beijing Neurosurgical Institute, Beijing 100050, China [2]Graduate School. Capital University of Medical Sciences, Beijing 100069, China

出　　处：《Neural Regeneration Research》2007年第5期276-280,共5页中国神经再生研究（英文版）

摘　　要：BACKGROUND： During formation of prolactin neoplasia, how cells and its structure in adenohypophysis affect prolactin cells should be further studied. Intermediate lobe can be regarded as a driving region to release prolactin （PRL） and may promote formation of prolactin neoplasia in pituitary anterior lobe. OBJECTIVE： To observe the effect of diethylstilbestrol （DES） on the expressions of μ and m-calpains in pituitary intermediate lobe of female Wistar rats. DESIGN： Observational contrast animal study. SETTING： Beijing Neurosurgical Institute. MATERIALS： A total of 21 female Wistar rats, 3 weeks old weighing 70-80 g were housed with free access to tap water and standard pellet food. They were kept in a CL-grade condition, at （24±1）℃ and a humidity of （55±5）%, and with a 12 hours day-night cycle. Caprine anti-μ- and m-calpains antibodies were provided by Santa Cruz Biotechnology, CA, USA; rabbit-anti-PRL antibodies by Dako, Denmark; rabbit-anti-ACTH antibody by Boster Company, Wuhan. METHODS： The experiment was carried out in Pathophysiological Department and Animal Laboratory, Beijing Neurosurgical Institute from August 2006 to January 2007. ① Rats were randomly divided into groups with 7 in each group, including vehicle control group, in which rats were injected intraperitoneally with sun-flower seed oil （1 mL/kg, twice a week） for 16 weeks; DES group, where animals were administered with DES （5 mg/kg, twice a week） for 16 weeks; DES ＋ vehicle control group, in which DES was administered for 12 weeks at the same dose with those in DES group, and then was discontinued and replaced by sun-flower seed oil （1 mL/kg, twice a week） for the following 4 weeks. ② At 16 weeks later, pituitary tissue was dealt with HE staining and PRL immunohistochemical examination to observe evoke of tumor; meanwhile, immunohistochemical examination was used to observe expression of PRL of pituitary anterior lobe, expressions of μ- and m-calpains of pituitary intermediate lobe and distrBACKGROUND： During formation of prolactin neoplasia, how cells and its structure in adenohypophysis affect prolactin cells should be further studied. Intermediate lobe can be regarded as a driving region to release prolactin （PRL） and may promote formation of prolactin neoplasia in pituitary anterior lobe. OBJECTIVE： To observe the effect of diethylstilbestrol （DES） on the expressions of μ and m-calpains in pituitary intermediate lobe of female Wistar rats. DESIGN： Observational contrast animal study. SETTING： Beijing Neurosurgical Institute. MATERIALS： A total of 21 female Wistar rats, 3 weeks old weighing 70-80 g were housed with free access to tap water and standard pellet food. They were kept in a CL-grade condition, at （24±1）℃ and a humidity of （55±5）%, and with a 12 hours day-night cycle. Caprine anti-μ- and m-calpains antibodies were provided by Santa Cruz Biotechnology, CA, USA; rabbit-anti-PRL antibodies by Dako, Denmark; rabbit-anti-ACTH antibody by Boster Company, Wuhan. METHODS： The experiment was carried out in Pathophysiological Department and Animal Laboratory, Beijing Neurosurgical Institute from August 2006 to January 2007. ① Rats were randomly divided into groups with 7 in each group, including vehicle control group, in which rats were injected intraperitoneally with sun-flower seed oil （1 mL/kg, twice a week） for 16 weeks; DES group, where animals were administered with DES （5 mg/kg, twice a week） for 16 weeks; DES ＋ vehicle control group, in which DES was administered for 12 weeks at the same dose with those in DES group, and then was discontinued and replaced by sun-flower seed oil （1 mL/kg, twice a week） for the following 4 weeks. ② At 16 weeks later, pituitary tissue was dealt with HE staining and PRL immunohistochemical examination to observe evoke of tumor; meanwhile, immunohistochemical examination was used to observe expression of PRL of pituitary anterior lobe, expressions of μ- and m-calpains of pituitary intermediate lobe and distr

关 键 词：DIETHYLSTILBESTROL PROLACTINOMA CALPAINS prolactin intermediate lobe 

分 类 号：R58[医药卫生—内分泌]