白藜芦醇抑制麻醉大鼠颈动脉窦压力感受性反射(英文)  

Resveratrol inhibits carotid sinus baroreceptor reflex in anesthetized rats

作　　者：薛红梅[1] 武宇明[1] 肖琳[1] 王茹[1] 王福伟[1] 何瑞荣[1] 

机构地区：[1]河北医科大学基础所生理室,河北石家庄050017

出　　处：《中国药理学与毒理学杂志》2007年第3期161-166,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金资助项目(304005);河北省自然科学基金资助项目(303467)~~

摘　　要：目的研究白藜芦醇的心血管保护作用是否与其对颈动脉窦压力感受器的作用有关。方法隔离灌流麻醉大鼠颈动脉窦区,同时记录动脉血压的变化,并绘制压力感受性反射的功能曲线。结果白藜芦醇(30,60和120μmol.L-1)隔离灌流颈动脉窦区,压力感受性反射机能曲线向右上方移位,曲线的最大斜率下降,反射性血压下降的幅度降低,阈压增加,其变化呈浓度依赖性。预先应用NO合酶抑制剂L-NAME100μmo.lL-1或钙通道开放剂BayK8644500nmol.L-1可消除白藜芦醇对压力感受器的抑制作用。用酪氨酸磷酸酶抑制剂正钒酸钠1mmol.L-1预处理对白藜芦醇抑制压力感受性反射的作用无影响。结论白藜芦醇对大鼠颈动脉窦压力感受器反射有抑制作用,此作用可能与局部NO的释放及减弱牵张敏感性通道的钙离子内流有关。AIM To study the relationship between cardioprotective effects of resveratrol and carotid sinus baroreflex （CSB）. METHODS The functional curve of the CSB was measured by recording changes in arterial pressure in anesthetized male rats with perfused isolated carotid sinus. RESULTS Resveratrol （30, 60 and 120 μmol·L^-1） inhibited the CSB, which shif- ted the functional curve of the baroreflex to the right and upward. There were a marked decrease in peak slope and a reflex decrease of blood pressure, and also an increase in threshold pressure. Changes of these parameters showed a concentration-dependent manner. Pretreatment with N^ω-nitro-L-arginine methylester （ 100 μmol·L^-1）, an inhibitor of nitric oxide synthase, and pretreatment with Bay K8644 （500 nmol· L^- 1 ）, an agonist of L-type calcium channel, could both eliminate the inhibitory effect of resveratrol on CSB. A potent inhibitor of tyrosine phosphatase sodium orthovanadate （1 mmol·L^-1） did not influence the effect of resveratrol on CSB. CONCLUSION Resveratrol inhibits carotid baroreflex, which may be mediated by the lo- cally released NO and decreased calcium influx.

关 键 词：白藜芦醇 颈动脉窦 压力感受器 

分 类 号：R285[医药卫生—中药学]