当归芍药散活性部位JD-30对淀粉样β蛋白片段25-35抑制大鼠海马脑片CA1区长时程增强的改善作用  

JD-30,an active constituent extracted from Danggui Shaoyao San,ameliorates amyloid beta-protein fragment 25-35 induced inhibition of long-term potentiation of CA1 area in rat hippocampal slices

作　　者：胡增峣[1] 杨胜[1] 周文霞[1] 张永祥[1] 尚玮玮[1] 乔善义[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国药理学与毒理学杂志》2007年第3期185-189,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家重点基础研究发展计划(973)项目(2004CB518907);国家自然科学基金项目(30500659)~~

摘　　要：目的研究当归芍药散改善学习记忆能力的物质基础和作用机理。方法采用细胞外微电极记录技术,记录大鼠海马脑片CA1区群峰电位(PS)幅值和高频刺激诱发LTP后PS的增幅。结果用含当归芍药散活性部位JD-30(25,50和100mg.L-1)的人工脑脊液灌流大鼠海马脑片,其CA1区的PS幅值无明显变化。用相同浓度的JD-30孵育海马脑片90min以上并持续灌流,其CA1区高频刺激后的PS增幅与空白对照组相比无明显差异。而用Aβ25-35200nmo.lL-1处理的海马脑片CA1区高频刺激后的PS增幅受到明显抑制;若同时给予Aβ25-35和上述浓度的JD-30处理海马脑片,CA1区高频刺激后的PS增幅较Aβ25-35组升高,其中JD-30100mg.L-1组的PS增幅达到正常对照组水平。提示JD-30对正常海马脑片CA1区的基础突触传递和LTP没有影响,但可改善Aβ25-35所抑制的LTP。结论JD-30可改善神经突触可塑性,拮抗Aβ对LTP的抑制作用可能是其益智机制之一。AIM To study the material base and mechanism of cognition enhancing effect of Danggui Shaoyao San （DSS, also named Tokishakuyaku-san in Japanese）. METHODS By using extracellular microelectrode recording technique, population spike （PS） and long-germ potentiation（LTP） of CA1 area in hippocampal slices were induced and recorded. RESLUTS Perfused with JD-30（25, 50 and 100 mg·L^-1）, an active constituent extracted from DSS, had no effect on PS amplitude of CA1 area in hippocampal slices. Incubated and perfused with the same concentrations of JD-30 had no effect on PS amplification of CA1 area after high frequency stimulation. Treatment with Aβ25-35 200 nmol· L^-1 significant depressed PS amplification of CA1 area, and the inhibition by Aβ25-35 was ameliorated by JD-30. Especially, JD-30 100 mg· L^-1 retrieved PS amplification to the normal group level. CONCLUSION JD-30 may improve synaptic plasticity, and it is possibly one of the mechanisms for its cognition enhancement action to ameliorate the inhibition of Aβ on LTP.

关 键 词：活性部位 当归芍药散 淀粉样Β蛋白 海马 长时程增强 微电极 

分 类 号：R285.5[医药卫生—中药学] R971[医药卫生—中医学]