邻烯丙基苯酚处理黄瓜种子控制土传腐霉菌和丝核菌侵染危害秧苗的效果  

Effect of o-Allylphenol on Controlling Cucumber Seedling Diseases Caused by Soil-borne Pathogens Pythium and Rhizoctonia with Seed Treatment

作　　者：曹锦[1] 周向阳[2] 李健强[1] 李永红[2] 侯红利[2] 张文华[1] 

机构地区：[1]中国农业大学植物病理学系,北京100094 [2]深圳市农作物良种引进中心,深圳518040

出　　处：《农药学学报》2007年第2期131-136,共6页Chinese Journal of Pesticide Science

基　　金：国家科技攻关项目(2004BA521B04);深圳市科技与信息局计划项目(S040161);教育部"新世纪优秀人才支持计划"(NCET-04-0128)资助

摘　　要：采用ISTA滤纸法检测得出：20%邻烯丙基苯酚悬浮种衣剂按1∶30-1∶90药种比（质量比）包衣处理“中农8号”和“长春密刺”黄瓜品种的种子,对黄瓜种子安全,种子发芽率分别达到95.75%-97.75%和80.00%-84.25%;PDA平板上检测药剂包衣处理对黄瓜种子带菌消毒效果显示,消毒处理效果达到66.67%-100%。菌丝生长速率法和染色处理结合显微技术实验证实,邻烯丙基苯酚对危害黄瓜秧苗的土传病原菌腐霉病菌Pythium aphanidermatum和立枯丝核菌Rhizoctonia solani具有较强的抑制作用,EC50值分别为27.59和34.16μg/mL;药剂处理使供试病菌的菌丝发生中空、解体断裂等异常现象。20%邻烯丙基苯酚悬浮种衣剂1∶30-1∶90包衣处理黄瓜种子后接种供试病菌,对腐霉病菌和立枯丝核菌的防病效果分别达到73.31%-100%和85.16%-100%;随着药剂包衣处理浓度的增大,邻烯丙基苯酚防治两种病原菌侵染危害黄瓜秧苗的效果提高,等效或者优于常规药剂福美双种子处理。Germination safety to cucumber seeds （two cultivars Zhongnong No. 8 and Changchun Mici） ,coated with 20% o-allylphenol seed coating formulation at the ratio of 1：30 - 1：90 （ratios of seed weight）was tested by ISTA＇s blotter paper method. The results showed that o-allylphenol seed treatment is safe to seed germination, the germination ratios for both cultivars were 95.75 % - 97.75 % and 80.00% -84.25% ,respectively. Based on the PDA test,the control effect of seed disinfection was up to 66. 67% - 100%. Hyphal growth rate test and microscopy demonstrated that o-allylphenol markedly inhibited the growth of two harmful soilborne pathogens, Pythium aphanidermatum and Rhizoctonia solani, with EC50 value ranging from 27.59 to 34.16 μg/mL. The o-allylphenol treatment resulted in abnormal appearance of fungal hypha, such as broken and cavity. When the cucumber seeds were treated with 20% o-allylphenol seed coating formulation as previous ratios before inoculated with P. aphanidermatum and R. solani, the disease control efficiency was 73.31% - 100% and 85.16% - 100% ,respectively. The control efficacy to both cucumber seedling diseases rose as the o-allylphenol concentration increased. The efficacy was equal or superior to that of regular dressing treatment with thiram.

关 键 词：邻烯丙基苯酚 腐霉病菌 立枯丝核菌 黄瓜种子 包衣处理 防病效果 

分 类 号：S482.292[农业科学—农药学]