防风胚状体发生发育中多糖的组织化学定位和提取  

The Histochemical Localization and Extraction of Polysaccharides during the Asexual Cmbryogenesis and Development of Saposhnikovia divaricata

作　　者：乔琦[1] 肖娅苹[2] 薛娴[1] 

机构地区：[1]河南科技大学农学院,洛阳471000 [2]陕西师范大学生命科学学院,西安710062

出　　处：《天然产物研究与开发》2007年第3期481-483,共3页Natural Product Research and Development

基　　金：国家十五攻关基金项目(2001BA701A35)

摘　　要：以防风胚状体的发生体系为材料,利用半薄切片技术研究了培养物中淀粉体的组织化学定位,并采用分光光度法测定了不同培养阶段多糖含量的动态变化。结果发现在胚性细胞内积累了大量的淀粉体;在4%蔗糖浓度培养基中培养的胚性愈伤组织多糖含量最高。研究表明多糖为胚性细胞的分化和发育直接提供了物质和能源;胚性愈伤组织可以推荐为药性成分多糖的提取材料之一。The asexual cmbryogenesis of Saposhnikovla divarlcata （Turcz.） Schischk. is chosen as the material to study the distribution of starch in cultures by semithin section and the change of polysaccharides by spectrophotometry in the different stages. The results showed that there were lots of starches in the embryogenic cell, and the highest content phase of polysaccharides was the embryogenic callus cultured in 4% sucrose medium. The studies indicate that the active metabolism of polysaccharides can provide intermediate products and energy for the embryogenic cell differentiation and development directly, and embryogenic callus can be recommended as one of the sources to extract polysaccharides.

关 键 词：防风 胚状体发生 淀粉体 多糖 

分 类 号：Q945[生物学—植物学] R93[医药卫生—生药学]