山仙颗粒对Lewis肺癌瘤株转染小鼠巨噬细胞和白介素12影响的实验研究  

Empirical study of effect of Shanxian granula on macrophage and interleukin-12 of mice with metastasis of Lewis lung cancer

作　　者：饶群阳 刘成高 徐基平 

机构地区：[1]湖南省衡阳市中医医院,湖南衡阳421001

出　　处：《现代中西医结合杂志》2007年第21期2985-2987,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的探讨山仙颗粒对Lewis肺癌瘤株转染的C57BL/6小鼠巨噬细胞(MΦ)和白介素12(IL-12)的影响。方法将40只小鼠随机分成4组,A组为空白组,B、C、D组均接种瘤株造模,24 h后A、B组以生理盐水灌胃,C、D组分别以治疗剂量的金龙胶囊和山仙颗粒混悬液灌胃,21 d后处死小鼠,检测MΦ与IL-12水平及其他各指标。结果C、D组小鼠出瘤时间、移植瘤质量、肺转移灶个数、去瘤去肺体质量、腹腔MΦ吞噬功能及IL-12的水平与B组小鼠比较均有显著性差异(P<0.01或0.05)。而C组与D组之间比较无显著性差异(P>0.05)。结论山仙颗粒能显著抑制C57BL/6小鼠Lewis肺癌的生长和自发性肺转移,其抗Lewis肺癌转移机制与体内MΦ被激活、吞噬功能加强及IL-12水平上调有关。Objective It is to investigate the effect of Shanxian granula on macrophage and interleukin- 12（IL- 12） of mice with metastasis of Lewis lung cancer. Methods 40 mice were divided into 4 groups randomly, A was the blank contrast group, and B, C, D were made into model groups. The next day after making model, each of group C and D was given Jin-Long Capsule and Shanxian granula separately, and group A and B were given normal saline. 21 days later, those mice were put to death, then tested the phagocytosis of macrophage and the level of IL - 12 and the other targets. Results The time of the tumor coming into being, the weight of the transplanted tumor and mice, the number of metastasis, the phagocytosis of macrophage and the level of IL- 12 of group C and D in contrast of those of B group, the meaning was prominent （P〈 0.01, P〈 0.05）, and group C almost hadn＇t difference with group D （P 〉 0.05）. Conclusion ShanXian granula can resist the growth and spontaneous metastasis of Lewis lung cancer. The mechanism of resisting metastasis maybe relate with strengthening phagocytosis of macrophage and heightening the level of IL- 12,

关 键 词：山仙颗粒 LEWIS肺癌 侵袭 转移 巨噬细胞 白细胞介素12 益气活血 

分 类 号：R965.2[医药卫生—药理学]