基于元分析的抗玉米丝黑穗病QTL比较定位  

Comparative QTL Mapping of Resistance to Sporisorium reiliana in Maize Based on Meta-analysis of QTL Locations

作　　者：吉海莲[1] 李新海[1] 谢传晓[1] 郝转芳[1] 吕香玲[1] 史利玉[1] 张世煌[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《植物遗传资源学报》2007年第2期132-139,共8页Journal of Plant Genetic Resources

基　　金：引进国际先进农业科学技术计划(2006-G3);国家"863"计划(2003AA207070)

摘　　要：以玉米遗传连锁图谱IBM2 2005 Neighbors为参考图谱,通过映射整合不同试验中的抗玉米丝黑穗病QTL,构建QTL综合图谱。在国内外种质中,共发现22个抗病QTL,分布在除第7染色体外的9条玉米染色体上。采用元分析技术,获得2个“一致性”抗病QTL,图距分别为8.79 cM和18.92cM。从MaizeGDB网站下载“一致性”QTL区间内基因和标记的原始序列;采用NCBI网站在线软件BLASTx通过同源比对在2个“一致性”QTL区间内初步获得4个抗病位置候选基因。借助比较基因电子定位策略,将69个水稻和玉米抗性基因定位于玉米IBM2图谱上,在2个“一致性”QTL区间内分别发现1个水稻抗性基因,初步推断为抗病位置候选基因。本文结果为抗玉米丝黑穗病QTL精细定位和分子育种提供了基础。The integration QTL map for Sporisorium reiliana resistance in maize was constructed by compiling a total of 22 QTL available for this trait with Genetic Map IBM2 2005 Neighbors as reference. These 22 QTL were found to be located on 9 chromosomes of maize with exception of chromosome 7. Using a meta-analysis method, 2 ＂consensus＂ QTL were identified on chromosomes 2 and 3 with map interval of 8.79cM and 18.92cM, respectively. The gene and marker sequences within 2 ＂ consensus＂ QTL were downloaded respectively from website Maize GDB, and 4 positional candidate genes were identified by comparing these sequences against resistance genes of other Poacease by internet software BLASTx. Using a synteny conservation approach based on comparative mapping between maize genetic map and rice physical map, a total of 69 rice and maize resistance genes were projected onto maize genetic map IBM2 2005 Neighbors, and one each rice resistance gene was found in 2 ＂consensus QTL＂. The results provided useful information for fine mapping QTL, gene cloning and molecular breeding for resistance to Sporisorium reiliana in maize.

关 键 词：玉米 丝黑穗病 数量性状位点 基因比较定位 

分 类 号：S513[农业科学—作物学]