α-淀粉酶和超氧化物歧化酶等位酶与水稻种子超干燥保存遗传完整性的研究  

Study on the Genetic Integrity of Ultra-dried Seed of Rice with Isozyme of α-Amy and SOD

作　　者：胡小荣[1] 陶梅[1] 卢新雄[1] 辛萍萍[1] 周红立[1] 

机构地区：[1]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京100081

出　　处：《植物遗传资源学报》2007年第2期228-230,共3页Journal of Plant Genetic Resources

基　　金：国家"十五"攻关(2001BA511B01);国家基础性工作资助项目

摘　　要：不同含水量的水稻种子在0℃和室温中保存40个月后,经发芽检测和а-淀粉酶、超氧化物歧化酶等位酶分析,结果表明,种子含水量降至3.0%以下,种子的耐贮性下降,发芽率降至61%以下,种子的遗传完整性受到影响。а-淀粉酶等位酶技术适合用于检测水稻种子超干燥保存的遗传完整性。Rice seed with different moisture content ranging from 2, 0% to 9.4% was stored at 0℃ and room temperature in Beijing for 40 months. The testing results of germination and isozyme of α-Amy and SOD showed that the storability of seed decreased when the water content decreased. The genetic integrity would be affected if the seed germination percentage was below 61%. α-Amy isozyme can be used to test the genetic integrity of rice seed with different moisture contents and germination percentage.

关 键 词：水稻种子 超干燥 保存 遗传完整性 等位酶 

分 类 号：S511[农业科学—作物学]