伊贝沙坦对血管紧张素Ⅱ所致心肌细胞蛋白质合成和肌球蛋白重链表达的影响  

Effect of irbesartan on cardiac hypertrophy and MHC gene expression induced by angiotensin II in rat

作　　者：陈爱兰[1] 陈敏生[2] 何兆初[1] 刘启才[3] 董颀[4] 

机构地区：[1]广州医学院第一附属医院心内科,510120 [2]广州医学院第二附属医院心内科 [3]广州医学院医学研究中心 [4]广州医学院生理教研室

出　　处：《现代临床医学生物工程学杂志》2006年第6期475-477,共3页Journal of Modern Clinical Medical Bioengineering

基　　金：国家自然科学基金(30440053)

摘　　要：目的观察伊贝沙坦对血管紧张素Ⅱ(AngⅡ)所致心肌细胞中蛋白质合成速率及肌球蛋白重链(MHC)基因表达改变的影响。方法以AngⅡ及伊贝沙坦分别或同时作用于培养的细胞。采用放射性同位素[3H]-leu掺入法检测培养心肌细胞蛋白质合成速率。应用荧光定量PCR方法检测心肌细胞心房利钠肽因子(ANF)以及α-MHC、β-MHC的表达。结果AngⅡ处理使心肌细胞中[3H]-Leu掺入增加(P<0.05),同时ANF mRNA的表达明显高于正常(P<0.05);α-MHC mRNA的表达显著低于正常(P<0.05),而β-MHC mRNA的表达显著高于正常(P<0.05),α-MHC/β-MHC的比值下降(P<0.05)。当伊贝沙坦与AngⅡ共同作用于培养的心肌细胞时,与AngⅡ组比较,[3H]-Leu的掺入明显下降(P<0.05),与正常组比无统计学意义(P>0.05);同时ANF的表达下降,与正常组比无统计学意义(P>0.05);心肌细胞中α-MHC的表达明显增高(P<0.05),而β-MHC mRNA的表达显著降低(P<0.05),α-MHC/β-MHC的比值上升(P<0.05)。结论伊贝沙坦能抑制AngⅡ所致的心肌细胞肥大和细胞中α-MHC向β-MHC表达的转换。Objective To investigate the effect of irbesartan on cardiac hypertrophy and myosin heary chain （MHC） gene expression induced by angiotensin Ⅱ （Ang Ⅱ ）in rats. Methods Cardiocytes of neonatal SD were cultured with Ang Ⅱ. Irbesartan was used to inhibit the effect of Ang Ⅱ. For assessing protein synthesis rate, ANF, β-MHC and α-MHC gene expression in cardiomyocyte, the methods of ^3H- Leucine incorporation and RT-QT-PCR were used. Results Ang Ⅱ could significantly increase ^3H -Leucine incorporation and the gene expression of ANF and β-MHC compared with control group （P 〈 0. 05, 0. 05, 0. 05 ）, but decrease α-MHC gene expression （ P 〈 0. 05 ）, accompanied the decrease of α-MHC/β-MHC value（P 〈0.05 ）. The effects of Ang Ⅱ were inhibited by irbesartan. The ^3H -Leucine incorporation and the gene expression of ANF and β-MHC were decreased in the co-stimulation with Ang Ⅱ and irbesartan, and no significant differences were found as compared with control group （ P 〉 0.05, 0. 05, 0. 05 ）, but the gene expression of α-MHC was significantly inhanced （ P 〈 0. 05 ）. Similarly the value of α-MHC/β-MHC was increased （P 〈 0. 05 ）. Conclusion In cultured neonatal cardiocytes, irbesartan can inhibit cardiocyte hypertrophy and the shift from α-MHC to β-MHC gene expression induced by AngⅡ.

关 键 词：伊贝沙坦 血管紧张素Ⅱ 心肌肥大 肌球蛋白重链 心房利纳肽因子 

分 类 号：R96[医药卫生—药理学]