应用蚕豆根尖细胞微核试验研究三氯甲烷与四氯化碳的遗传毒性  

THE GENOTOXICITY STUDY OF CHCl 3 AND CCl 4 USINGMICRONUCLEUS TEST IN VICIA FABA ROOT TIP CELLS

作　　者：金志玉 马松科 杨儒道[1] 

机构地区：[1]云南省卫生防疫站

出　　处：《癌变．畸变．突变》1997年第3期150-154,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：本文采用蚕豆根尖细胞微核测试法，对三氯甲烷与四氯化碳的遗传毒性及其交互作用进行了研究。结果表明：三氯甲烷（０．５０３－５０．３０２μｍｏｌ／Ｌ）与四氯化碳（０．０１９５－１．９５０１μｍｏｌ／Ｌ）均分别显著诱发蚕豆根尖细胞微核的形成（Ｐ＜０．０１），并呈现明显的剂量效应。三氯甲烷与四氯化碳同时处理蚕豆根尖细胞时，诱发微核率仍然显著高于对照组，但表现一定的交互拮抗作用。结果提示，三氯甲烷与四氯化碳对生物遗传物质具有显著损伤效应。本文还探讨了饮用水中二种受试物质最高容许浓度的安全性问题。The genotoxicity and interaction of CHCl 3 and CCl 4 were analyzed by micronucleus test in Vicia faba root tip cells.CHCl 3(0.503-50.302 μmol/L)and CCl 4 0.0195-1.9501μmol/L)significantly induced micronuclei in the Vicia Faba cells(P<0.01),respectively.A significant dose-response was found.A significant effects on micronucleus induction and interaction were observed when the solution containing 2 chemicals was used to treat the Vicia Faba cells.2 chemicals showed some antagonistic effect.The results indicated that CHCl 3 and CCl 4 possess significant genotoxicity.We've also approached the safety of maximum acceptable concentration of CHCl 3 and CCl 4 in drinking water.

关 键 词：三氯甲烷 四氯化碳 遗传毒性 交互作用 

分 类 号：R995[医药卫生—毒理学] R992[医药卫生—药学]