正己烷致大鼠过氧化损伤及肝细胞凋亡的实验研究  

Experimental study on the effect of n-hexane on lipid peroxidation damage and hepatic cell apoptosis in rats

作　　者：齐宝宁[1] 唐国慧[1] 易建华[1] 郭剑锋[1] 苗江丽[1] 

机构地区：[1]西安交通大学医学院劳动卫生与环境卫生教研室,陕西西安710061

出　　处：《中国工业医学杂志》2007年第3期177-179,共3页Chinese Journal of Industrial Medicine

摘　　要：目的研究正己烷(n-hexane)对大鼠的脂质过氧化损伤和肝细胞凋亡的影响。方法40只雄性SD大鼠随机分成5组,即阴性对照组、染毒组(75、150、300 mg/kg)和阳性对照组(环磷酰胺),每组8只。经腹腔注射染毒4周后,检测肝组织匀浆超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力,血清还原型谷胱甘肽(GSH)和丙二醛(MDA)含量;用流式细胞仪检测肝细胞凋亡情况。结果随着染毒剂量的增加,肝组织匀浆中SOD、GSH-Px活力、血清GSH含量降低,而血清MDA含量增大,组间比较差异有统计学意义(P<0.05或P<0.01),流式细胞仪检测结果显示,肝细胞凋亡率(AV+/PI-)组间比较差异有统计学意义(P<0.01),与染毒剂量作相关分析,相关系数为0.913,无明显的相关性(P>0.05)。结论正己烷可引起或增强机体氧自由基反应,导致脂质过氧化损伤,引起肝细胞凋亡和坏死。Objeetive To study the effect of n-hexane on lipid peroxidation damage and hepatic cell apoptosis in rats. Method 40 SD male rats were randomly divided into 5 groups, namely negative control group, 75 mg/kg n-hexane group, 150 mg/kg n-hexane group, 300 mg/kg n-hexane group and positive control group （cyclophosphamide）, 8 rats for each group. Administrations had been given by intraperitoneal injection for four weeks, then the activities of GSH-Px and SOD in hepatic homogenate and the levels of GSH and MDA in serum apoptosis rate of the hepatic cell were detected, respectively. Result The results showed that with the increasing of n-hexane dosage, the activities of GSH-Px and SOD in hepatic homogenate and the concentration level of GSH in serum were reduced, while the serum MDA level was increased, there were statistic significances among the groups （ P 〈 0.05 or P 〈 0.01 ）. The detection of hepatocytic apoptosis rate by FCM showed that there were also some differences existed among the groups, but no correlation between dosage and apoptosis rate was found （ r = 0.913, P 〉 0.05 ）. Conclusion n-Hexane could induce or enhance the lipid peroxdation damage, hepatocytic apoptosis or necrosis in rats.

关 键 词：正己烷 脂质过氧化 细胞凋亡 大鼠 

分 类 号：O623.11[理学—有机化学]