湖北省柑桔裂皮类病毒的检测及序列分析  

Detection and Analysis of Genomic RNA Sequence of Citrus exocortis viroid from Hubei Province

作　　者：何云蔚[1] 洪霓[1] 徐文兴[1] 王国平[1] 

机构地区：[1]华中农业大学植物科技学院

出　　处：《华中农业大学学报》2007年第3期289-292,共4页Journal of Huazhong Agricultural University

基　　金：科技部863项目(2001AA241142)资助

摘　　要：以表现柑桔裂皮病典型症状的罗伯逊脐橙为材料,采用双向电泳、RT-PCR和RNA斑点杂交及组织印迹杂交的方法,对柑桔裂皮类病毒进行了检测,结果表明RT-PCR和2种核酸杂交技术均可用于该类病毒的有效检测。对该类病毒全长RNA的RT-PCR产物进行了克隆和序列分析,该分离株（命名为CEVd-HB分离株）的全长RNA由371个核苷酸组成,与已报道的其它不同来源分离株相似性为90%～99%。Citrus exocortis viroid （CEVd） in Citrus sinensis Osbeck cv. Robertson, which showed typical symptoms of CEVd infection, were detected by bi-directional PAGE electrophoresis, RT-PCR, RNA dot-blotting and tissue-printing hybridization. Results showed that RT-PCR and two hybridization methods were effective for the detection of the viroid. The RT-PCR products from the full-length genomic RNA of the viroid（named as CEVd-HB） were cloned. Results of sequence analysis indicated that the full-length genomic RNA of the viroid consist of 371 nucleotide acids and had similarities ranging from 90% to 99% with reported isolates of the viroid.

关 键 词：柑桔裂皮类病毒 检测 克隆 序列分析 

分 类 号：S432.41[农业科学—植物病理学] S666[农业科学—农业昆虫与害虫防治]