猪腔前卵泡的体外培养  

In vitro Culture of Porcine Preantral Follicles

作　　者：靳双星[1] 张桂枝[1] 李跃民[2] 

机构地区：[1]郑州牧业工程高等专科学校畜牧系,郑州450011 [2]西南大学动物科技学院,重庆400716

出　　处：《华中农业大学学报》2007年第3期344-347,共4页Journal of Huazhong Agricultural University

基　　金：河南省重大科技攻关项目(0422011100)资助

摘　　要：为确定不同基础培养液和培养方法对猪腔前卵泡体外培养的影响,试验采用了M199和NCSU23两种基础培养液及96孔培养板法、凹窝培养法和颗粒细胞铺层培养法对猪腔前卵泡进行体外培养。结果表明:培养在M199的腔前卵泡生长显著快于培养在NCSU23的腔前卵泡,培养在该2组的猪腔前卵泡前5 d的生长速度都极显著地高于后5 d的生长速度,而腔前卵泡的成腔率和存活率没有明显差异;培养在凹窝培养体系的腔前卵泡生长速度显著低于96孔培养板法,但凹窝培养体系可以显著地增加腔前卵泡的存活率。同时证明,颗粒细胞铺层培养法对腔前卵泡生长没有显著影响。To investigate the effect of different basic mediam and methods on the in vitro culture of porcine preantral follicles,two basic medium of M199 and NCSU23 and three of 96-well plates, hollowness and granulose cells coating culture systems were adopted in culturing porcine preantral follicles in vitro. Results showed that preantral follicles cultured in M199 medium grew faster than those in NCSU23. The follicular growth rate during the first 5 days of culture was higher than that during the latter 5 days in both of the two culture-medium groups. However, antrum formation and follicular livability had no difference between the two groups. The follicular growth rate in hollowness culture system was lower than that in 96-well plates, but hollowness culture system increased the livability of preantral follicles. Granulose cells coating culture system was proved to have no influence on porcine preantral follicles development.

关 键 词：猪 腔前卵泡 体外培养 

分 类 号：Q813.7[生物学—生物工程]