黄芪多糖对实验性乳腺炎山羊血清及乳中生化指标的影响  

Effect of Astragalus Polysaccharide on Biochemical Index of Blood Serum and Milk of Goat Experimental Mastitis

作　　者：钟凯[1] 杨国宇[1] 刘仪[2] 王艳玲[1] 邹思湘[2] 陈伟华[2] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]南京农业大学动物医学院,南京210095

出　　处：《华中农业大学学报》2007年第3期353-356,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30671533);河南省杰出人才创新基金(0121000300)资助

摘　　要：选择同处于泌乳初期的6头睢宁白山羊为试验动物,于左、右乳区分别灌注黄芪多糖和生理盐水5 mL,连续灌注5 d,第6天注入大肠杆菌内毒素诱导产生山羊实验性乳腺炎。于注入内毒素前1 h及灌注后3、6、9、24 h取颈静脉血和采集乳样,测定乳液及血清中N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)活力及丙二醛含量(MDA)等指标。结果表明:与灌注内毒素前相比,灌注生理盐水的对照乳区乳中的NAGase活力在注入内毒素后9 h时达到峰值(23.95 U/mg),而灌注黄芪多糖的试验乳区乳中的NAGase活力6 h时达到峰值(28.29 U/mg),24 h时乳中NAGase活力已下降到灌注内毒素以前的水平。山羊乳导管注入内毒素后,2种处理的乳区乳中的总抗氧化能力显著下降,乳中MDA的含量显著升高,SOD活力显著下降;2种处理的乳区乳之间比较无显著差异。Six Suining White goats in their early lactation were used in this study. The mammary right quarters of 6 goats were infused with about 5 mL of Astragalus polysaccharide （APS）. Left quarters were used as controls and infused with 5 mL of physiological saline solution （PSS）. The administration of APS had been continuing for five consecutive days. On the sixth day, after morning milking, the mammary quarters were infused with LPS according to a dosage of 50μg per kilogram body weight. At varying intervals before and after infusion （-1,3,6,9,and 24 h）, quarter milk samples and blood samples from jugular vein were collected. The dynamics of N-acetyl-β-D-glucosaminidase （NAGase） activity, alkaline phosphatase （AKP） activity, SOD activity, and MDA content were determined. The results showed that the NAGase activity in the milk from quarters administered with PSS had reached the peak （23.95 U/mg） at the 9th hour. While the NAGase activity in the milk from quarters administered with APS had reached the peak （28.29 U/mg） at 6th hour and declined to the normal level at 24th hour. Compared to the pre-infusion of LPS,T-AOC in the milk from quarters administered both PSS and APS had decreased significantly,MDA content significantly higher.

关 键 词：黄芪多糖 山羊 乳腺炎 内毒素 

分 类 号：S853.51[农业科学—临床兽医学]