Biosynthesis and Characterization of Polyhydroxyalkanoate Copolyesters in Ralstonia eutropha PHB-4 Harboring a Low-Substrate-Specificity PHA Synthase PhaC2ps from Pseudomonas stutzeri 1317  

作　　者：陈晶瑜 张磊 陈金春 陈国强 

机构地区：[1]MOE Laboratory of Protein Science, Department of Biological Sciences and Biotechnology, Tsinghua University,Beijing 100084, China [2]M-ulti-disciplinary Research Center, Shantou University, Shantou 515063, China

出　　处：《Chinese Journal of Chemical Engineering》2007年第3期391-396,共6页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China （No.30225001, No.20334020）. The authors are very grateful to Prof. A. Steinbiichel of the University of Miinster in Germany for the generous donation of strain R. eutropha PHB 4.

摘　　要：A series of polyhydroxyalkanoate(PHA)copolymers consisting of short-chain-length(SCL)and medium-chain-length(MCL)3-hydroxyalkanoate(3HA)monomers were synthesized in the recombinant Ralstonia eutropha PHB - 4 harboring a low-substrate-specificity PHA synthase PhaC2Ps from Pseudomonas stutzeri 1317. These polyesters,whose monomer compositions varied widely in chain length,were purified and characterized by acetone fractionation,nuclear magnetic resonance(NMR),gel-permeation chromatography(GPC),and differential scanning calorimetry(DSC).This was the first time that the physical properties of PHA copolymers polymerized by PhaC2Ps were characterized.The results indicated that the variation in MCL 3HA contents did not have an obvious influence on the molecular weights of these PHA copolymers but was effective in changing their physical properties. The variation in the thermal property of PHA copolymers with 3-hydroxyoctanoate(3HO)content was also inves- tigated in this study.A series of polyhydroxyalkanoate（PHA）copolymers consisting of short-chain-length（SCL）and medium-chain-length（MCL）3-hydroxyalkanoate（3HA）monomers were synthesized in the recombinant Ralstonia eutropha PHB - 4 harboring a low-substrate-specificity PHA synthase PhaC2Ps from Pseudomonas stutzeri 1317. These polyesters,whose monomer compositions varied widely in chain length,were purified and characterized by acetone fractionation,nuclear magnetic resonance（NMR）,gel-permeation chromatography（GPC）,and differential scanning calorimetry（DSC）.This was the first time that the physical properties of PHA copolymers polymerized by PhaC2Ps were characterized.The results indicated that the variation in MCL 3HA contents did not have an obvious influence on the molecular weights of these PHA copolymers but was effective in changing their physical properties. The variation in the thermal property of PHA copolymers with 3-hydroxyoctanoate（3HO） content was also investigated in this study.

关 键 词：polyddroxybutyrate PHA synthase physical property Pseudomonas stutzeri 

分 类 号：TQ325[化学工程—合成树脂塑料工业]