锰对大鼠生精细胞凋亡与增殖的影响  

Effect of manganese on apoptosis and proliferation in spermatogenic cell of rats

作　　者：张先平[1] 才秀莲[1] 王乾兴 刘连[1] 

机构地区：[1]遵义医学院组织胚胎学教研室,贵州遵义563003 [2]中国医学科学院中国协和医科大学

出　　处：《毒理学杂志》2007年第3期176-179,共4页Journal of Toxicology

摘　　要：目的研究氯化锰(MnCl2.4H2O)对大鼠生精细胞凋亡与增殖的影响。方法健康雄性SD大鼠24只随机分为3组,每组8只。染锰组分别腹腔注射15和30 mg/kg MnCl2.4H2O,对照组腹腔注射等容生理盐水,1次/d,每周5 d,共4周,停药2周后处死大鼠取材。透射电子显微镜观察凋亡细胞形态;原位末端脱氧核苷酸转移酶标记(terminaldeoxynucleotidyl transferase-mediated dUTP nick end labeling,TUNEL)技术检测生精细胞凋亡;免疫组化S-P法检测生精细胞增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)表达。结果(1)电子显微镜下各组大鼠均可见生精细胞凋亡表现。(2)15和30 mg/kg染锰组大鼠睾丸生精细胞凋亡指数(apoptosis index,AI)分别为(1.05±0.19)%和(1.80±0.21)%,均明显高于对照组(0.48±0.01)%(P<0.01),且AI随染锰剂量的增加而升高(P<0.01)。(3)生精细胞增殖指数(proliferating index,PI)在15和30 mg/kg染锰组分别为(22.68±2.62)%和(14.25±2.33)%,均明显低于对照组(33.25±3.59)%(P<0.01),且PI随染锰剂量的增加而显著降低(P<0.01)。(4)AI与PI呈显著负相关(r=-0.88,P<0.01)。结论锰可使大鼠生精细胞凋亡增加及PCNA表达减弱。Objective To study the effect of manganese chloride on apoptosis and proliferation in spermatogenic cell of rats. Methods 24 healthy male SD rats were randomly divided into three groups and there were 8 rats in every group. The rats of control group were given intraperitoneal injections with normal saline and the exposure groups were administered respectively at the doses of 15 or 30 mg/kg manganese chloride （MnCl2 ·4H2 O） for 4 weeks. All rats were injected by intraperitoneal injection once everyday and 5 times in a week. Then all rats were sacrificed and their testes were extracted after 2 weeks. Apoptotic morphologic changes of the spermatogenic cell were observed by transmission electron microscope. The number of apoptosis spermatogenic cell was examined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） technique. In addition, the expression of proliferating cell nuclear antigen （PCNA） of spermatogenic cell was measured by immunohistochemistry method. Results 1. The apoptotic morphologic changes of spermatogenic cell were observed in every group. 2. The apoptosis indexes （AI） of spermatogenic cell in exposure groups were respectively （ 1.05 ± 0.19） %, （ 1.80 ± 0.21 ） % and significant increases of AI were observed when compared with those of the control groups （0.48 ± 0.01 ） % （ P 〈 0.01 ）. Moreover, it was increased with dosage of manganese （ P 〈 0.01 ）. 3. Proliferating indexes （PI） in exposure groups were respectively （22.68 ± 2.62）%, （14.25 ± 2.33 ）% and were more lower than those of control groups （33.25 ± 3.59）% （ P 〈 0.01 ）. Moreover, it was decreased with dosage of manganese （ P 〈 0.01 ）. 4.There existed a significant negative correlation between AI and PI of rat spermatogenic cell （ r = - 0.88, P 〈 0.01 ）. Conclusion Manganese could promote apoptosis and reduce the expression of PCNA in spermatogenic cell of rat.

关 键 词：锰 生精细胞 凋亡 增殖细胞核抗原 

分 类 号：R697[医药卫生—泌尿科学]