无筛选标记基因高油酸花生高效载体的构建  

A construction of high efficient vector for high oleic and selectable marker free peanut,Arachis hypogaea

作　　者：李桂民[1] 宋凯[1] 冯凯[1] 闻珊珊[1] 刘立侠[1] 许守民[1] 

机构地区：[1]东北师范大学生命科学学院

出　　处：《东北师大学报（自然科学版）》2007年第2期97-102,共6页Journal of Northeast Normal University(Natural Science Edition)

基　　金：吉林省科技发展计划项目(2003070620040209-1)

摘　　要：提高油酸的相对含量,增加油酸/亚油酸(O/L)的比值,已成为目前油料作物品质改良的重要内容.ahFAD2B是编码花生微粒体ω6-脂肪酸脱氢酶的主要基因,根据双链RNA介导的基因沉默原理,设计和构建了可以抑制花生种子油酸脱氢酶基因ahFAD2B表达的载体,从而提高了油酸的含量,改善了花生油脂肪酸的组成.进一步将该构建组装到一个双右侧边界(DRB)的共转化载体中,用该载体来转化花生,以期从转基因后代中筛选无抗生素标记基因的高油酸花生材料.One of the major goals for oilseed research is to improve the nutritive value of oilseeds by increasing the ratio of oleic acid to linoleic acid.In this study, employed hpRNA-mediated gene-silencing techniques,and genetically constructed a vector to down-regulate the expression of key fatty acid desaturease gene of peanut,ahFAD2B,encoding microsomal ω 6-desaturease and modify The fatty acid composition of peanut oil（with high oleic oil content）.The cassette was assembled to a ＂double right border＂ vector,by which marker genes are eliminated efficiently soon after transformation. The vector will be used to transform the peanut tissue. This strategy provides an effective approach for selectable marker free and high oleic peanut.

关 键 词：花生转化 基因沉默 高油酸 发卡RNA 双右侧边界载体 

分 类 号：Q782[生物学—分子生物学]