水貂肠炎细小病毒PCR检测方法的建立和应用  

Establishment and Application of PCR for Detection of Mink Enteritis Virus

作　　者：张海玲[1] 闫喜军[1] 柴秀丽[1] 吴威[1] 易立[1] 罗国良[1] 田洪宇[1] 邵西群[1] 王凤雪[1] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109

出　　处：《特产研究》2007年第2期1-3,共3页Special Wild Economic Animal and Plant Research

基　　金：科技部科研院所社会公益研究专项(2005DIB4J048)

摘　　要：根据GenBank上已发表的水貂肠炎细小病毒基因序列,在其编码VP2结构蛋白基因的高度保守区内,设计合成1对特异性引物,建立水貂肠炎细小病毒PCR诊断方法。经过敏感性及特异性检测,该引物能与MEV标准株和地方分离株核酸发生特异性结合,扩增出一段长度为570bp的DNA片段,而与CDV、ADV等病毒的PCR反应均呈阴性。设计的该对引物可用于MEV的临床检测。Two primers were designed and synthesized according to the nucleocapasid gene sequence of mink enteritis virus from GenBank , and used to detect mink enteritis virus. Special products of 570bp size were amplified by these primers from MEV vaccine and suspected cases but not from CDV, CAV and ADV strains.The results indicated that the PCR assay of MEV was proved to be specific and sensitive. It is feasible method for rapid diagnosis of MEV.

关 键 词：水貂病毒性肠炎 PCR 引物 

分 类 号：S858.92[农业科学—临床兽医学]